Data · dataset · 2025
Preparation and Application of Monoclonal Antibody Against 3-Methoxydopamine
Listed in ScienceDB
Objective This study aims to prepare monoclonal antibodies against 3-methoxydopamine and verify the antibody performance via magnetic microparticle chemiluminescence method.
Description
Method
The 3-methoxydopamine antigen was designed and synthesized.Immune New Zealand white rabbits with 3-methoxydopamine coupled to bis-succinimidyl suberate sodium salt (BS3) and Keyhole limpet hemocyanin (KLH) to obtain an antibody library.High-affinity monoclonal antibodies were obtained using solid- phase selection strategy based on phage display technology.Antibody performance was verified by coating magnetic particles with biotin-labeled antibodies and combining them with horseradish peroxidase-labeled antigen.Result Through phage display technology, 20 monoclonal antibodies with high affinity for 3-methoxydopamine were obtained.
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After ELISA titer determination, monoclonal antibody 4354# with the best titer and gradient was selected. Biotin-conjugated monoclonal antibody 4354# was used as the coating antibody, and horseradish peroxidase-labeled 3-methoxydopamine served as the enzyme-labeled antigen. The magnetic microparticle chemiluminescence method verified that monoclonal antibody 4354# can be used for competitive detection of 3-methoxydopamine in urine.
The test results showed a significant positive correlation with the HPLC assay results (R² = 0.9021).Conclusion A monoclonal antibody against 3-methoxytyramine was successfully isolated using phage display technology. It was then validated via a magnetic microparticle chemiluminescence method for its ability to quantitatively detect 3-methoxytyramine in urine through a competitive method. This work has laid the foundation for the development of materials for 3-methoxytyramine diagnostic kits.
Links
Where it is published
- DOI doi.org/10.57760/sciencedb.j00217.08135 ↗
DOI / persistent id · from scidb cn
Catalogue records · 1
- OAI-PMH record scidb.cn/oai?verb=GetRecord&metadataPrefix=oai_dc&identifier=10.57760%2… ↗
metadata API · from scidb cn
Topics
- From keywords
- Chemistry · Earth & Environmental Science · Engineering · Humanities · Life Sciences · Social Science
Provenance · 1 source records, 11 field assertions
| Source | Key | Last seen | Raw |
|---|---|---|---|
| ScienceDB | 10.57760/sciencedb.j00217.08135 | 11 d ago | JSON v1 |
| Field | Assertion | Extractor | Evidence |
|---|---|---|---|
| access_level | source · scidb cn | connector:scidb_cn@1.0.0 | |
| concepts[field].local:field:chemistry | mapping · scidb cn | connector:scidb_cn@1.0.0 | |
| concepts[field].local:field:earth-environmental | mapping · scidb cn | connector:scidb_cn@1.0.0 | |
| concepts[field].local:field:engineering | mapping · scidb cn | connector:scidb_cn@1.0.0 | |
| concepts[field].local:field:humanities | mapping · scidb cn | connector:scidb_cn@1.0.0 | |
| concepts[field].local:field:life-sciences | mapping · scidb cn | connector:scidb_cn@1.0.0 | |
| concepts[field].local:field:social-science | mapping · scidb cn | connector:scidb_cn@1.0.0 | |
| description | source · scidb cn | connector:scidb_cn@1.0.0 | /metadata/dc/description |
| license_text | source · scidb cn | connector:scidb_cn@1.0.0 | |
| publication_date | source · scidb cn | connector:scidb_cn@1.0.0 | |
| title | source · scidb cn | connector:scidb_cn@1.0.0 | /metadata/dc/title |