Diversity and metabolic abilities of Antarctic bacteria

These were SSSI 16 and SSSI 17. Samples were also collected from around Casey Station. SSSI 16 and SSSI 17 are now known as Antarctic Specially Protected Areas.

The new designation for SSSI 16 and 17 respectively is ASPA 135 and ASPA 136. The following work was completed as part of this project: 1) Obtained some bacteria isolates from antarctic soil and sediment, using common and selective microbiological media. 2) From the micropore membranes, we extracted genomic DNA, PCR the 16S rRNA gene, cloned and sequence the gene to determine bacterial species. Water samples were filtered through micropore membranes to concentrate bacteria.

The download file contains data on bacterial diversity in meltlake water and sediment from two sites around Casey station, ASPA 135 and ASPA 136. ASPA 135 meltlake (66.28251 degrees S, 110.54192 degrees E) Temperature 5.8 degrees C, pH 6.06, salinity 0 Sample collection date 27 December 2002 ASPA 136 meltlake (66.25131 degrees S, 110.53978 degrees E) pH 7.35, salinity 2.8 Sample collection date 28 December 2002 The download files contain a series of accession numbers for the Genbank Database.

The actual data can be obtained from Genbank.