Data · dataset · 2025
AutoSpectral: 40-colour ID7000 dataset
Listed in Teesside University Research Data Repository
Healthy human PBMC and buffy coat samples, cryopreserved and stained with a 40-colour flow cytometry panel.
Description
Acquired on a 5-laser Sony ID7000 spectral instrument. Unmixed using WLSM on the Sony software or using AutoSpectral.
WLSM: Sony software unmixed using weighted least squares WLS: AutoSpectral unmixed using weighted least squares scAF: AutoSpectral unmixed using weighted least squares and per-cell (single cell) autofluorescence extraction perCellFluor: AutoSpectral unmixed using weighted least squares, per-cell autofluorescence extraction and per-cell fluorophore spectral optimization. Slow = exact recomputation of the unmixing matrix per variant, Medium = rank-one updating, Fast = approximation using the difference in spectrum between variants and the abundance of the endmember (fluorophore).
Links
Where it is published
- DOI doi.org/10.17632/2kvc98hc65.2 ↗
DOI / persistent id · from researchdata tees ac uk
Catalogue records · 1
- OAI-PMH record data.mendeley.com/oai?verb=GetRecord&metadataPrefix=oai_dc&identifier=oai%3Adata… ↗
metadata API · from researchdata tees ac uk
Topics
- From keywords
- Earth & Environmental Science · Engineering · Humanities · Life Sciences · Social Science
Provenance · 1 source records, 10 field assertions
| Source | Key | Last seen | Raw |
|---|---|---|---|
| Teesside University Research Data Repository | oai:data.mendeley.com/2kvc98hc65.2 | 11 d ago | JSON v1 |
| Field | Assertion | Extractor | Evidence |
|---|---|---|---|
| access_level | source · researchdata tees ac uk | connector:researchdata_tees_ac_uk@1.0.0 | |
| concepts[field].local:field:earth-environmental | mapping · researchdata tees ac uk | connector:researchdata_tees_ac_uk@1.0.0 | |
| concepts[field].local:field:engineering | mapping · researchdata tees ac uk | connector:researchdata_tees_ac_uk@1.0.0 | |
| concepts[field].local:field:humanities | mapping · researchdata tees ac uk | connector:researchdata_tees_ac_uk@1.0.0 | |
| concepts[field].local:field:life-sciences | mapping · researchdata tees ac uk | connector:researchdata_tees_ac_uk@1.0.0 | |
| concepts[field].local:field:social-science | mapping · researchdata tees ac uk | connector:researchdata_tees_ac_uk@1.0.0 | |
| description | source · researchdata tees ac uk | connector:researchdata_tees_ac_uk@1.0.0 | /metadata/dc/description |
| license | source · researchdata tees ac uk | connector:researchdata_tees_ac_uk@1.0.0 | /metadata/dc/rights |
| publication_date | source · researchdata tees ac uk | connector:researchdata_tees_ac_uk@1.0.0 | |
| title | source · researchdata tees ac uk | connector:researchdata_tees_ac_uk@1.0.0 | /metadata/dc/title |