QUB-1475: A novel bioactive peptide from the skin secretion of frog Hylarana Guentheri

As a consequence, the emergence of the antimicrobial peptide (AMP) derived from the skin secretion of amphibians has promoted the development of novel potential clinical therapeutics. Therefore, a novel potential bioactive peptide QUB-1475 derived from the frog species Hylarana guentheri was chosen here to get thoroughly studied. <br>In this research, the novel temporin-like peptide, with the sequence: FLQHIIGALGFIF-NH2, was discovered in the skin secretion of Hylarana guentheri.

This thesis aimed to isolate and characterise the structure of the novel peptide QUB-1475 through “shotgun” molecular cloning, reverse-phase High-Performance Liquid Chromatography (RP-HPLC) and Matrix Assisted Laser Desorption/Ionization Time of Flight Mass Spectrometry (MALDI-TOF MS) analysis. Then the peptide was chemically-synthesised and evaluated through a series of bioactivity assays.<br>This peptide displayed no antimicrobial activity on S. aureus, E. coli and C. albicans conducted in this research.