Transcriptomic profiling of murine liver, lung, and adipose functional cells across aging and chronic disease models [RNA-Seq]

To identify organ-specific SASP factors associated with mesenchymal drift in liver, lung, and adipose diseases, we performed RNA‑seq on primary hepatocytes (mPHs) from STAM mice, pulmonary fibroblasts (mPLFs) from bleomycin‑induced IPF mice, and adipose‑derived MSCs (mADSCs) from obese mice, as well as from normal and naturally aged mice. Compared with controls, the upregulated genes shared across each disease model and aged mice were defined as mesenchymal drift‑associated SASP (MD‑SASP) signatures.

We identified 10 hepatic, 8 pulmonary, and 8 adipose MD‑SASP factors, including liver‑specific Il‑8/Tgfb3/Edn1/Cxcl16, lung‑specific Il6/Igfbp7, and adipose‑specific Fgf7/Timp1. These factors can serve as candidate indicators for evaluating cellular interstitial senescence in future studies.