Table · dataset · 2025
RNA-seq analysis of mycobacteria stress response to microgravity
Listed in NASA Data Portal
The aim of this work is to determine whether mycobacteria have enhanced virulence during space travel and what mechanisms they use to adapt to microgravity.
Description
M. marinum and LHM4 were grown in high aspect ratio vessels (HARV) in a rotary cell culture system (RCCS) under normal gravity (NG) or low shear simulated microgravity (MG). To determine the effect of MG on the stress responses activated by the growth conditions, we used RNAseq to examine what genes were expressed.
For RNAseq, the bacteria are harvested, RNA isolated and converted DNA (cDNA), and the cDNA sequenced. Using bioinformatics, the amount of expression of the different M. marinum genes were compared between the NG and MG samples. To make sure that we were examining only gene expression changes due to MG, only bacteria in early exponential growth were used in the RNAseq studies.
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Triplicate NG and MG cultures were used to generate samples of bacteria grown for ~40 hrs. We also grew triplicate cultures for 4 days and then diluted them again and grew them for another ~40 hrs so we could examine gene expression from bacteria exposed for a longer time. In summary, this study determined that waterborne mycobacteria alter their growth, expression of stress responses, and their sensitivity to oxidizing conditions when subjected to growth under MG.
Links
Get the data
- Resource lsda.jsc.nasa.gov/scripts/experiment/exper.aspx?exp_index=13484 ↗
download · from data nasa gov
- Resource purl.bioontology.org/ontology/MESH/D019910 ↗
download · from data nasa gov
- Resource osdr.nasa.gov/bio/repo/data/studies/OSD-90 ↗
download · from data nasa gov
Where it is published
- NASA Data Portal dataset page data.nasa.gov/dataset/rna-seq-analysis-of-mycobacteria-stress-response-to-mi… ↗
landing page · from data nasa gov
- DOI doi.org/10.26030/3wcg-5t20 ↗
DOI / persistent id · from data nasa gov
Catalogue records · 1
- CKAN API data.nasa.gov/api/3/action/package_show?id=0f9da38f-a0ac-4f66-932f-53fbc4d3d… ↗
metadata API · from data nasa gov
Topics
- From keywords
- Astronomy & Astrophysics · Earth & Environmental Science · Life Sciences · Ocean & Atmospheric Science · Physics
- Inferred from text
- Evolutionary biology 68% · RNA sequencing 65%
Provenance · 1 source records, 13 field assertions
| Source | Key | Last seen | Raw |
|---|---|---|---|
| NASA Data Portal | 0f9da38f-a0ac-4f66-932f-53fbc4d3d147 | 12 d ago | JSON v1 |
| Field | Assertion | Extractor | Evidence |
|---|---|---|---|
| concepts[field].anzsrc:group:3104 | enrichment · data nasa gov | taxonomy-embedding@1.0.0 | title+keywords+description (68%) |
| concepts[field].local:field:astronomy | mapping · data nasa gov | connector:data_nasa_gov@1.0.0 | |
| concepts[field].local:field:earth-environmental | mapping · data nasa gov | connector:data_nasa_gov@1.0.0 | |
| concepts[field].local:field:life-sciences | mapping · data nasa gov | connector:data_nasa_gov@1.0.0 | |
| concepts[field].local:field:ocean-atmospheric | mapping · data nasa gov | connector:data_nasa_gov@1.0.0 | |
| concepts[field].local:field:physics | mapping · data nasa gov | connector:data_nasa_gov@1.0.0 | |
| concepts[modality].local:modality:rna-seq | enrichment · data nasa gov | keyword-concept-rules@1.0.0 | title+description (65%) |
| created_date | source · data nasa gov | connector:data_nasa_gov@1.0.0 | |
| description | source · data nasa gov | connector:data_nasa_gov@1.0.0 | /notes |
| license_text | source · data nasa gov | connector:data_nasa_gov@1.0.0 | |
| publication_date | source · data nasa gov | connector:data_nasa_gov@1.0.0 | |
| title | source · data nasa gov | connector:data_nasa_gov@1.0.0 | /title |
| updated_date | source · data nasa gov | connector:data_nasa_gov@1.0.0 |