Table 1_The adaptive landscape of the miR482 gene family in tomato is rugged.xlsx

Successful miR:target interactions rely on high sequence complementarity, usually permitting few to no mismatches. Exploring the concept of an adaptive landscape, we investigated how individual nucleotide changes in the miR482 gene family may alter the targeted repertoire of disease resistance gene (R-genes) in tomato.

We predicted the target scores for all mutants accessible through individual point mutations in silico and compared them to the target scores of the original miR482 genes. Most mutations lead to a loss of targeting or lower likelihood of targeting. Only a few mutants showed improved target scores or gained new R-gene targets.