Omics · study · 2026
Transcriptional co-regulator Med15 is required to maintain functional maturation in adult β-cells
Listed in NCBI GEO
Transcriptional coregulators, such as the Mediator complex, partner with transcription factors to regulate gene transcription throughout all stages of life.
Description
Subunit Med15 is required for pre-natal development and post-natal maturation of pancreatic β-cells. However, whether Med15 continues to play a role in β-cell function after maturation is complete, and throughout adulthood, is unknown.
To investigate the role of Med15 in β-cells post-maturation, we induced a β-cell specific Med15 knockout at six weeks age in male and female mice. Ablation of Med15 led to glucose intolerance and impaired insulin secretion. RNA-sequencing revealed downregulation of β-cell maturation markers.
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Further, we implanted insulin pellets into knockout mice to lower blood glucose and performed RNA-seq to validate that the transcriptional changes were a direct consequence of the Med15 loss and not due to hyperglycemia in the knockout mice. This study shows that Med15 is required after weaning and throughout life to maintain functional β-cell maturity.
Links
Get the data
- GEO FTP directory ftp.ncbi.nlm.nih.gov/geo/series/GSE343nnn/GSE343956 ↗
download · from NCBI GEO
Where it is published
- GEO accession page ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE343956 ↗
landing page · from NCBI GEO
Documentation and papers
- PRJNA1514006 ncbi.nlm.nih.gov/bioproject/PRJNA1514006 ↗
project · from NCBI GEO
Topics
- Stated by source
- Expression profiling by high throughput sequencing · Mus musculus
- From keywords
- Life Sciences
- Inferred from text
- Genetics 70% · RNA sequencing 65%
Provenance · 1 source records, 9 field assertions
| Source | Key | Last seen | Raw |
|---|---|---|---|
| NCBI GEO | GSE343956 | 14 d ago | JSON v1 |
| Field | Assertion | Extractor | Evidence |
|---|---|---|---|
| access_level | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[field].anzsrc:group:3105 | enrichment · NCBI GEO | taxonomy-embedding@1.1.0 | title+keywords+description (70%) |
| concepts[field].local:field:life-sciences | mapping · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[method].geo_series_type:expression-profiling-by-high-throughput-sequencing | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /gdstype |
| concepts[modality].local:modality:rna-seq | enrichment · NCBI GEO | keyword-concept-rules@1.0.0 | title+description (65%) |
| concepts[organism].NCBITaxon:10090 | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /taxon |
| description | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /summary |
| publication_date | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| title | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /title |