THE ROLE OF ABSCISIC ACID AND COLD ADAPTATION IN IMPROVING THE CRYOPRESERVATION EFFICIENCY OF PYROPIA HAITANENSIS CONCHOCELIS

To explore whether abscisic acid (ABA) and cold adaptation can improve the cryopreservation efficiency of Porphyra sensu lato, the free living conchocelis of Pyropia haitanensis were treated with ABA (100 μmol/L) for 48h at 20°C, light intensity of 40 μmol·photons/(m2·s) with photoperiod 12L﹕12D or cultured in dark at 4°C for 6 days before cryopreservation at –80°C. The effects of cryopreservation were evaluated by continuous measurement of algal survival rate after frozen, the maximum light quantum efficiency (Fv/Fm) of PSII and the expression of cold adaptative related genes.