Assessing bioactivity of peptide QUB2003 from the skin secretion of the Waxy monkey tree frog Phyllomedusa sauvagii

Subsequently, the predicted mature peptide had its structure confirmed by mass spectrometry and was then chemically-synthesised using solid phase peptide synthesis to provide adequate material for functional research. Functional tests employed included antimicrobial assays, haemolytic assays and anticancer cell assays.<br><br>The synthetic replicate of the peptide QUB2003, exhibited antimicrobial activity with MIC values against the Gram-positive bacterium, Staphylococcus aureus, the Gram-negative bacterium, Escherichia coli and the yeast, Candida albicans, of 32 µM, 256 µM and 32 µM, respectively, EC50=214.3 µM which possessed relatively strong haemolytic activity.

The MB435, PC3, H157, U251MG cells were thus unaffected in growth by QUB2003, cells proliferations were 125.24%, 102.87%, 135.70%, 78.48%, respectively.