Identification of GLIS2 direct target genes in kidney

To obtain insights into the role of GLIS2 in the regulation of normal kidney functions and in the development of NPHP, we investigated GLIS2 direct target genes in kidney. Due to a lack of suitable GLIS2 antibody for chromatin immunoprecipitation (ChIP), we generated Glis2-HA mice in which HA tag was inserted before the TGA stop codon of GLIS2.

Thereby, endogenous GLIS2 protein is expressed as a fusion protein with HA tag. GLIS2 ChIP-Seq was performed using HA antibody with kidneys from Glis2-HA mice. GLIS2 cistrome analysis identified many of genes associated with inflammation, ECM, and cell adhesion as direct targets by GLIS2.