Data · dataset · 2025
Differential biological effects of ultra-high dose rate irradiation on BEAS-2B and A549 cells
Listed in ScienceDB
Objective Comparative analysis of the differential effects of Ultra High Dose Rate (UHDR) and conventional dose rate electron beam irradiation on normal and tumor cells.
Description
Method
Using a 6MeV electron beam linear accelerator, normal cell line (BEAS-2B) and tumor cell line (A549) were irradiated separately to establish a control group, conventional (CONV) irradiation group, and UHDR irradiation group; Using CCK8 experiment to detect the effects of different dose rates of irradiation on the survival ability of normal cells and tumor cells; Determine oxidative damage by measuring the levels of SOD and MDA in cells after irradiation; Immunofluorescence assay is used to detect double stranded DNA breaks and repair in cells, while flow cytometry is used for cell cycle detection.Results After exposure to 6 Gy and 15 Gy of CONV and UHDR irradiation, the survival rate of BEAS-2B cells in the UHDR group was significantly higher than that in the CONV group (F=12.73, P<0.05; F=13.92, P<0.05; F=38.07, P<0.05; F=38.94, P<0.05), and the survival rate of BEAS-2B cells as normal cells was higher than that of A549 cells (F=8.198, P<0.05; F=19.21, P<0.05; F=34.97, P<0.05; F=19.24, P<0.05); After CONV irradiation, the SOD activity and MDA content of BEAS-2B and A549 cells were significantly changed.
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The SOD activity of BEAS-2B cells changed slightly after UHDR irradiation, and the difference was not statistically significant compared with the non-irradiated group. The SOD activity of A549 cells in the UHDR group was not statistically different from that in the CONV irradiation group. The MDA content of BEAS-2B cells in the UHDR irradiation group was lower than that in the CONV group (F=88.98， P<0.05； F=63.70， P<0.05； F=34.27， P<0.05； F=95.16， P<0.05 ).
Compared with the CONV irradiation group, there was no significant difference in the MDA content of A549 cells after UHDR irradiation; Compared with CONV, after UHDR irradiation, BEAS-2B cells showed stable G2 phase arrest at 24-72 hours, while A549 cells showed a high proportion of G1 phase arrest at 4 hours, but cell cycle collapse at 72 hours; After 4 hours of irradiation, the UHDR group showed less formation of gamma H2AX foci in BEAS-2B cells compared to the CONV group, and the gamma H2AX foci significantly decreased after 24 hours.
After UHDR irradiation, the number of DNA double-strand breaks was reduced, and they were repaired more quickly.Conclusion After UHDR irradiation, the radiation damage to lung epithelial-like cells BEAS-2B in terms of survival, oxidative damage, cell cycle, and DNA damage repair is less than that of conventional dose rate irradiation.
Links
Where it is published
- DOI doi.org/10.57760/sciencedb.zhfsyxyfhzz.00040 ↗
DOI / persistent id · from scidb cn
Catalogue records · 1
- OAI-PMH record scidb.cn/oai?verb=GetRecord&metadataPrefix=oai_dc&identifier=10.57760%2… ↗
metadata API · from scidb cn
Topics
- From keywords
- Earth & Environmental Science · Engineering · Humanities · Life Sciences · Social Science
- Inferred from text
- Cancer 65%
Provenance · 1 source records, 11 field assertions
| Source | Key | Last seen | Raw |
|---|---|---|---|
| ScienceDB | 10.57760/sciencedb.zhfsyxyfhzz.00040 | 12 d ago | JSON v1 |
| Field | Assertion | Extractor | Evidence |
|---|---|---|---|
| access_level | source · scidb cn | connector:scidb_cn@1.0.0 | |
| concepts[disease].local:disease:cancer | enrichment · scidb cn | keyword-concept-rules@1.0.0 | title+description (65%) |
| concepts[field].local:field:earth-environmental | mapping · scidb cn | connector:scidb_cn@1.0.0 | |
| concepts[field].local:field:engineering | mapping · scidb cn | connector:scidb_cn@1.0.0 | |
| concepts[field].local:field:humanities | mapping · scidb cn | connector:scidb_cn@1.0.0 | |
| concepts[field].local:field:life-sciences | mapping · scidb cn | connector:scidb_cn@1.0.0 | |
| concepts[field].local:field:social-science | mapping · scidb cn | connector:scidb_cn@1.0.0 | |
| description | source · scidb cn | connector:scidb_cn@1.0.0 | /metadata/dc/description |
| license | source · scidb cn | connector:scidb_cn@1.0.0 | /metadata/dc/rights |
| publication_date | source · scidb cn | connector:scidb_cn@1.0.0 | |
| title | source · scidb cn | connector:scidb_cn@1.0.0 | /metadata/dc/title |