supporting data for "CRISPR-based functional screening identifies <i>ITGAV</i> as a therapeutic target for 5-fluorouracil resistance in colorectal cancer"

The data support a research project that combines CRISPR screening, public gene-expression dataset analysis, clinical sample analysis, quantitative PCR, Western blotting, immunohistochemistry, cell proliferation and colony-formation assays, wound-healing and migration assays, flow-cytometric apoptosis analysis, drug-response and combination-index analyses, and in vivo tumor models, including a patient-derived xenograft model.

The study evaluates ITGAV expression and function in colorectal cancer cells and 5-FU-resistant derivatives and investigates whether ITGAV knockdown or pharmacological inhibition with cilengitide can improve cellular responses to 5-FU. The deposited files include spreadsheet data, GraphPad Prism analysis files, flow-cytometry data, statistical reports, an R analysis script, and supporting figure materials. Collectively, these data provide experimental evidence for understanding the association between ITGAV and 5-FU resistance and for evaluating ITGAV as a potential therapeutic target in colorectal cancer.</p>