Data · dataset · 2026
A bioactive peptide from the skin secretion of the Red-eyed Leaf Frog, Agalychnis callidryas
Listed in UCL Research Data Repository
Natural products, as rich and varied resources for the discovery of novel drugs, have been essential for human healthcare systems throughout history.
Description
A variety of bioactive components with unique structures and functions isolated from the skin secretion of amphibians, especially peptides, have enormous potential for medical purposes. <br><br>Here, a peptide from the phylloseptin family was identified from the Red-eyed Leaf Frog, Agalychnis callidryas, by molecular cloning.
This peptide was named as QUB-1541 based on its molecule weight. Then the mature peptide was successfully synthesised by Tribute® 2-channel peptide synthesiser. Reversed-phase high- performance liquid chromatography (RP-HPLC) was used for purification and matrix-assisted laser desorption ionisation time-of-flight (MALDI-TOF) MS was used to confirm its molecular mass.
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QUB-1541 has better inhibitory activity against gram-positive bacteria than gram-negative bacteria. The MIC for S. aureus is 8 μM while for E. coli is 512 μM. Also, QUB-1541 inhibits the growth of yeast, C. albicans and the MIC is 128 μM. The results of MBC show that QUB-1541 has no bactericidal effect on E. coli and C. albicans at the highest concentration. However, QUB-1541 kills S. aureus at a lower level of 16 μM, while the haemolysis is weak.
QUB-1541 was only effective against cancer cells, PC-3, U251MG and H157 at the highest concentration of 10-4 M. Thus, QUB-1541 has the value of subsequent research.<br><br><i>Thesis embargoed until 31st October 2024 </i><br>
Links
Where it is published
- DOI doi.org/10.17034/32628225.v1 ↗
DOI / persistent id · from rdr ucl ac uk
Catalogue records · 1
- OAI-PMH record api.figshare.com/v2/oai?verb=GetRecord&metadataPrefix=oai_dc&identifier=oai%3Af… ↗
metadata API · from rdr ucl ac uk
Topics
- From keywords
- Earth & Environmental Science
- Inferred from text
- Cancer 75%
Provenance · 1 source records, 6 field assertions
| Source | Key | Last seen | Raw |
|---|---|---|---|
| UCL Research Data Repository | oai:figshare.com:article/32628225 | 13 d ago | JSON v1 |
| Field | Assertion | Extractor | Evidence |
|---|---|---|---|
| concepts[disease].local:disease:cancer | enrichment · rdr ucl ac uk | keyword-concept-rules@1.0.0 | title+description (75%) |
| concepts[field].local:field:earth-environmental | mapping · rdr ucl ac uk | connector:rdr_ucl_ac_uk@1.0.0 | |
| description | source · rdr ucl ac uk | connector:rdr_ucl_ac_uk@1.0.0 | /metadata/dc/description |
| license_text | source · rdr ucl ac uk | connector:rdr_ucl_ac_uk@1.0.0 | |
| publication_date | source · rdr ucl ac uk | connector:rdr_ucl_ac_uk@1.0.0 | |
| title | source · rdr ucl ac uk | connector:rdr_ucl_ac_uk@1.0.0 | /metadata/dc/title |