Data · dataset · 2026
Identification and in <i>vitro</i> characterization of QUB-3358: a novel antimicrobial peptide from <i>Amolops torrentis</i> skin secretion
Listed in ZivaHub and Deakin Research Online and DMU Figshare — shown once because both records carry DOI 10.17034/32641866.v1
Faced with the challenges of the increasing proliferation of antibiotics and the continuous evolution of super bacteria, antimicrobial peptides (AMPs) are being explored as potential new treatments.
Description
Amphibian skin secretions contain a large number of AMPs, which are an essential host defence mechanism against bacteria. This paper utilised the freeze-dried skin secretions of the frog, Amolops torrentis, to identify a biosynthetic peptide precursor cDNA sequence, encoding a peptide named, QUB-3358, using the "shotgun" cloning method.
QUB-3358 belongs to Brevinin-2 peptide family structurally, and the mature peptide sequence was synthesised using solid-phase peptide synthesis (SPPS) technology and purified by reverse-phase high-performance liquid chromatography (RP-HPLC). The molecular mass of the peptide was determined by MALDI-TOF mass spectrometry. Its structure was simulated, and its physicochemical properties were analysed by PEPFOLD and HeliQuest on-line programmes.
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Finally, antibacterial, haemolytic and anti-proliferative assays were performed to evaluate the biological activity of the peptide. The biological activity testing demonstrated that QUB-3358 was antimicrobial and exhibited MICs against S. aureus, E. coli, and C. albicans of 8 μM, 16 μM, and 32 μM, respectively. It was almost non-haemolytic within the effective antibacterial concentration with only 9% at 128 μM. In addition, the IC50 of QUB-3358 against H838 lung cancer cells was 20.35 μM. Its anti-proliferative activity on MRC-5 normal lung cells (IC50: 20.41 μM) is the same as that on H838 lung cancer cells (IC50: 20.35 μM).
In conclusion, QUB-3358 exhibited broad-spectrum antimicrobial activity, had low haemolysis, and possessed anti-proliferative properties against cancer cells, highlighting its significant potential as a novel therapeutic agent.<br><br><i>Thesis is embargoed until 31 December 2030.</i>
Links
Where it is published
- DOI doi.org/10.17034/32641866.v1 ↗
DOI / persistent id · from zivahub uct ac za
Catalogue records · 1
- OAI-PMH record api.figshare.com/v2/oai?verb=GetRecord&metadataPrefix=oai_dc&identifier=oai%3Af… ↗
metadata API · from zivahub uct ac za
Topics
- From keywords
- Earth & Environmental Science · Earth & Environmental Science · Earth & Environmental Science
- Inferred from text
- Cancer 75% · Mass spectrometry 75%
Provenance · 3 source records, 10 field assertions
| Source | Key | Last seen | Raw |
|---|---|---|---|
| ZivaHub | oai:figshare.com:article/32641866 | 13 d ago | JSON v1 |
| Deakin Research Online | oai:figshare.com:article/32641866 | 13 d ago | JSON v1 |
| DMU Figshare | oai:figshare.com:article/32641866 | 13 d ago | JSON v1 |
| Field | Assertion | Extractor | Evidence |
|---|---|---|---|
| access_level | source · zivahub uct ac za | connector:zivahub_uct_ac_za@1.0.0 | |
| concepts[disease].local:disease:cancer | enrichment · zivahub uct ac za | keyword-concept-rules@1.0.0 | title+description (75%) |
| concepts[field].local:field:earth-environmental | mapping · zivahub uct ac za | connector:zivahub_uct_ac_za@1.0.0 | |
| concepts[field].local:field:earth-environmental | mapping · dro deakin edu au | connector:dro_deakin_edu_au@1.0.0 | |
| concepts[field].local:field:earth-environmental | mapping · figshare dmu ac uk | connector:figshare_dmu_ac_uk@1.0.0 | |
| concepts[modality].local:modality:mass-spectrometry | enrichment · zivahub uct ac za | keyword-concept-rules@1.0.0 | title+description (75%) |
| description | source · zivahub uct ac za | connector:zivahub_uct_ac_za@1.0.0 | /metadata/dc/description |
| license_text | source · zivahub uct ac za | connector:zivahub_uct_ac_za@1.0.0 | |
| publication_date | source · zivahub uct ac za | connector:zivahub_uct_ac_za@1.0.0 | |
| title | source · zivahub uct ac za | connector:zivahub_uct_ac_za@1.0.0 | /metadata/dc/title |