The RNA and protein landscapes of mouse brain organoids

Mouse brain organoids reproduce the main steps of neurodevelopment in vivo and differentiate more rapidly than human organoids, making them scalable for screening applications. However, mouse brain organoids remain poorly defined.

Here, we showed that the transcriptome of mouse brain organoids developed for three weeks is close to that of a neonatal mouse brain. Strikingly, organoids reproduced the majority of alternative splicing and polyadenylation site events that define a neural identity in vivo. Proteomics revealed that most changes in mRNA expression were translated into proteins.