Data · dataset · 2026
<p>The expression of <i>nhr-68</i> restored pathogen susceptibility.</p>
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<p><b>(A)</b> Representative fluorescence micrographs of <i>nhr-68(gk708);nhr-68p::nhr-68::gfp</i> animals. <i>n</i> = 30; data are representative of four independent experiments.
Description
Fluorescence images were captured using 400-ms exposures. Scale bars indicate 100 µm in the left two panels and 50 µm in the right two panels. <b>(B)</b> qRT–PCR analysis of the immune gene <i>clec-60</i> in wild-type, <i>nhr-68(gk708)</i> or <i>nhr-68(gk708);nhr-68p::nhr-68</i> animals fed on <i>E. coli</i>.
Data are presented as the mean ± SD from three independent biological experiments. <i>N</i> = 3; <i>n</i> = 200 animals per condition per biological replicate. ***<i>p</i> < 0.001, one-way ANOVA with Tukey’s multiple-comparison test. <b>(C)</b> Survival of wild-type N2, <i>nhr-68(gk708)</i> or <i>nhr-68(gk708);nhr-68p::nhr-68</i> animals on <i>P. aeruginosa</i> at 25 °C. Mean survival times were 2.07, 2.83, and 2.35 days, respectively.
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Data are presented from three independent experiments, with 90 animals per condition per experiment (<i>N</i> = 3; <i>n</i> = 90 animals per condition per experiment). Wild-type vs. <i>nhr-68(gk708)</i>, <i>p</i> < 0.0001; Wild-type vs. <i>nhr-68(gk708); nhr-68p::nhr-68</i>, <i>p</i> = 0.0002; <i>nhr-68(gk708)</i> vs. <i>nhr-68(gk708); nhr-68p::nhr-68</i>, <i>p</i> = 0.0202. <b>(D)</b> <i>P. aeruginosa</i> occupancy indexes for WT, <i>nhr-68(gk708)</i> and <i>nhr-68(gk708);nhr-68p::nhr-68</i> animals at 8 h and 12 h after seeding.
The occupancy index was calculated as (<i>N</i><sub>on</sub> lawn/<i>N</i><sub>total</sub>). Data are presented as mean ± SD from three independent experiments (<i>N</i> = 3). The data underlying this Figure can be found in <a href="plosbiology.org/article/info:doi/10.1371/journal.pbio.3004026#pbio.3004026.s009" target="_blank">S1 Data</a>.</p>
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- DOI doi.org/10.1371/journal.pbio.3004026.g002 ↗
DOI / persistent id · from zivahub uct ac za
Catalogue records · 1
- OAI-PMH record api.figshare.com/v2/oai?verb=GetRecord&metadataPrefix=oai_dc&identifier=oai%3Af… ↗
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- Biochemistry and cell biology · Bioinformatics and computational biology · Caenorhabditis elegans · Chemistry · Earth & Environmental Science · Ecology · Genetics · Immunology · Life Sciences · Medicine & Health · Psychology & Behavioral Science · Research, science and technology policy
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Provenance · 1 source records, 18 field assertions
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