Unravelling Zebrafish Heart Regeneration at Single-Cell Level

In contrast, zebrafish exhibits remarkable cardiac regeneration capacity. Several cell types and signaling pathways have been reported to participate in this process.

However, a comprehensive analysis of how different cells and signals interact and coordinate to regulate cardiac regeneration is unavailable. We collected major cardiac cell types from zebrafish and performed high-precision single-cell transcriptome analyses during development and post-injury regeneration. We revealed heterogeneity as well as the molecular progress of cardiomyocytes during these processes, and identified a subtype of atrial cardiomyocyte exhibiting a stem-like state which may transdifferentiate into ventricular cardiomyocytes during regeneration.