Effect of the Stability of the Poly(gamma-Glutamic Acid)-ACP Dispersion System on Biomimetic Mineralization of Type I Collagen.

X-ray diffraction (XRD), Fourier transform infrared (FTIR) spectroscopy and thermogravimetry (TG) were used to analyse the mineralized collagen scaffold composition.Results: The PGA-ACP solutions with γ-PGA of different MWs and concentrations had different stabilities and type I collagen mineralization. Except for the 100 kDa group, which neither stabilized the supersaturated calcium phosphate solution nor induced intrafibrillar mineralization, the groups stabilized the solutions for at least 12 h and induced different intrafibrillar mineralization patterns and degrees.

Conclusion: In our system, the PGA-ACP solution stability and occurrence of intrafibrillar mineralization are directly correlated. Thus, we suspect that the same correspondence exists in other biomimetic mineralization systems and that a relatively stable supersaturated calcium phosphate solution may be a necessary condition for intrafibrillar mineralizationFigure and the original data of them.The data plotting software is Origin 2024b, while the figures are stitched and annotated using sai2.