Data · dataset · 2026
<p>Mechanism and functional activity of V1WT_06, V1WT_41, and VA14_26.</p>
Listed in UCL Research Data Repository
<p>Neutralisation of sarbecoviruses through entry via different host ACE2s.
Description
Neutralisation of <b>A)</b> Rc-o319 and <b>B)</b> RhGB07 was measured against entry via <i>Rh. cornutus</i> ACE2 and <i>Rh. alcyone</i> ACE2, respectively, which were transiently expressed in HEK293T/17 cells. <b>C)</b> Neutralisation of WIV-1 was compared between entry via hu-ACE2 (dotted line and closed symbols) and <i>Rh. alcyone</i> ACE2 (full line and closed symbols).
Neutralisation was measured using HEK 293T cells transiently expressing either <i>Human</i> ACE2 or <i>Rh. alcyone</i> ACE2. For A-C, mean values are plotted with error bars showing ± standard deviation (s.d.). <b>D)</b> Ability of IgGs to inhibit the interaction between cell surface ACE2 (including hu-ACE2, <i>Rh. cornutus</i> ACE2 and <i>Rh. alcyone</i> ACE2) and soluble RBD (including SARS-CoV-2, WIV-1, Rs4231, RhBG07, and Rc-o319 RBDs).
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IgGs were pre-incubated with fluorescently labelled RBD before addition to ACE2 expressing cells. N.B = No binding of IgG to the RBD. The percentage reduction in mean fluorescence intensity is reported.
Experiments were performed in duplicate. <b>E)</b> Antibody dependant cellular cytotoxicity (ADCC) of IgGs measured through the surface expression of CD107a on activated NK cells (NK-92 cells expressing CD16) [<a href="plosbiology.org/article/info:doi/10.1371/journal.pbio.3003882#pbio.3003882.ref041" target="_blank">41</a>]. HIV-1 specific neutralising antibody, PGT128 is used as a negative control [<a href="plosbiology.org/article/info:doi/10.1371/journal.pbio.3003882#pbio.3003882.ref031" target="_blank">31</a>].
The data underlying this Figure can be found in <a href="plosbiology.org/article/info:doi/10.1371/journal.pbio.3003882#pbio.3003882.s010" target="_blank">S1 Data</a>.</p>
Links
Where it is published
- DOI doi.org/10.1371/journal.pbio.3003882.g004 ↗
DOI / persistent id · from rdr ucl ac uk
Catalogue records · 1
- OAI-PMH record api.figshare.com/v2/oai?verb=GetRecord&metadataPrefix=oai_dc&identifier=oai%3Af… ↗
metadata API · from rdr ucl ac uk
Topics
- From keywords
- Earth & Environmental Science · Ecology · Evolutionary biology · Immunology · Infectious diseases · Life Sciences · Medicine & Health · Microbiology · Social Science · Virology
Provenance · 1 source records, 15 field assertions
| Source | Key | Last seen | Raw |
|---|---|---|---|
| UCL Research Data Repository | oai:figshare.com:article/34048942 | 11 d ago | JSON v1 |
| Field | Assertion | Extractor | Evidence |
|---|---|---|---|
| access_level | source · rdr ucl ac uk | connector:rdr_ucl_ac_uk@1.0.0 | |
| concepts[field].anzsrc:field:310706 | mapping · rdr ucl ac uk | vocabulary-mapper@1.0.0 | keywords['Virology'] |
| concepts[field].anzsrc:field:320211 | mapping · rdr ucl ac uk | vocabulary-mapper@1.0.0 | keywords['Infectious Diseases'] |
| concepts[field].anzsrc:group:3103 | mapping · rdr ucl ac uk | vocabulary-mapper@1.0.0 | keywords['Ecology'] |
| concepts[field].anzsrc:group:3104 | mapping · rdr ucl ac uk | vocabulary-mapper@1.0.0 | keywords['Evolutionary Biology'] |
| concepts[field].anzsrc:group:3107 | mapping · rdr ucl ac uk | vocabulary-mapper@1.0.0 | keywords['Microbiology'] |
| concepts[field].anzsrc:group:3204 | mapping · rdr ucl ac uk | vocabulary-mapper@1.0.0 | keywords['Immunology'] |
| concepts[field].local:field:earth-environmental | mapping · rdr ucl ac uk | connector:rdr_ucl_ac_uk@1.0.0 | |
| concepts[field].local:field:life-sciences | mapping · rdr ucl ac uk | connector:rdr_ucl_ac_uk@1.0.0 | |
| concepts[field].local:field:medicine-health | mapping · rdr ucl ac uk | connector:rdr_ucl_ac_uk@1.0.0 | |
| concepts[field].local:field:social-science | mapping · rdr ucl ac uk | connector:rdr_ucl_ac_uk@1.0.0 | |
| description | source · rdr ucl ac uk | connector:rdr_ucl_ac_uk@1.0.0 | /metadata/dc/description |
| license | source · rdr ucl ac uk | connector:rdr_ucl_ac_uk@1.0.0 | /metadata/dc/rights |
| publication_date | source · rdr ucl ac uk | connector:rdr_ucl_ac_uk@1.0.0 | |
| title | source · rdr ucl ac uk | connector:rdr_ucl_ac_uk@1.0.0 | /metadata/dc/title |