Table 1_Performance comparison and clinical validation of two rapid direct identification and antimicrobial susceptibility testing approaches for pathogens isolated from positive blood cultures in children.docx

In terms of AST, using the standard AST result as reference, the HBL rapid method yielded an EA of 95.6% and a CA of 98.3%, with minor error (mE), major error (ME), very major error (VME), and bias rates of 1.1%, 0.4%, 0.1%, and -2.3%, respectively. Similarly, the rapid method with short-term culture showed comparable efficacy (EA, 96.5%; CA, 98.8%; mE, 1.0%; ME, 0.2%; VME, 0.1%; bias, -0.3%). Crucially, the mean turnaround times (TATs) for the HBL rapid method (44.7 hours) and the rapid method with short-term culture (45.9 hours) were significantly shorter than the standard method (68.4 hours), representing an approximate 24-hour reduction in BC workflow.</p>Conclusions<p>The two rapid methods exhibit exceptional accuracy and reliability for species identification and AST directly from positive blood cultures, which can significantly shorten the TAT for BSI diagnosis, offering clinicians to optimize timely and appropriate clinical treatment.</p>