<p>Autoantibody levels are elevated in NZBxW/F1 mice with age and curli treatment.</p>

<p>Serum was collected from (<b>A, D</b>) pre-onset, (<b>B, E</b>) early-disease, and (<b>C,F</b>) late-disease NZBxW/F1 mice 24 hours after each weekly injection of curli purified from <i>Salmonella</i> Typhimurium biofilms or PBS for untreated controls. <b>(A-C)</b> ELISA assays detected anti-dsDNA antibodies in serum diluted (<b>A, B</b>) 1:250 or (<b>C</b>) 1:500 (<b>A-B,</b> N = 8; <b>C,</b> N = 15). Week -1 denotes baseline samples collected from each group one week before the first injection (Week 0). <b>(D-F)</b> ELISA assays detected anti-C1q antibodies in serum diluted 1:50, normalized to the <b>O.</b>D. of positive control MRL/lpr serum, indicated by the top dotted line, and negative controls without serum are indicated by the bottom dotted line (<b>D-E,</b> pre-bleed and Week 4 not assayed due to insufficient serum volume for the assay).

Statistics: (<b>A-B, D-E</b>) Two-way ANOVA, or <b>(C, F)</b> Mixed-effects model, with Tukey’s multiple comparisons, comparisons within legends indicating overall column factor, (*p < 0.05, **p < 0.01).</p>