Data · dataset · 2022
qF3 Analysis Code
Listed in Borealis and Agri-environmental Research Data Dataverse — shown once because both records carry DOI 10.5683/sp3/qydedh
Provided here: -Scripts for Performing quantitative Fast FLIM FRET (qF3) Analysis.
Description
Organized into folders by steps (1-13) to run for each biological replicate (Step1_Replicate_Analysis) and then for all combined replicates (Step2_CombineReps_Analysis). -script used to calculate G factor (for which our original data can be provided upon request) -Example Master platemap. -LICENSE file for all code here. -Author: Nehad Hirmiz (Nehad.Hirmiz@gmail.com) Instructions: -Download all files here and extract .Zip. -Install MATLAB Version R2020a with toolboxes: Signal Processing, Curve Fitting, Image Processing. -IFF starting with INO FLIM Hyperspectral data
- then contact lead for INO software package including (Release_r10357 package): INO FHS Acquisition, INO_FHS_Analysis, INO_FHS_Batch Analysis -Follow instructions to run these codes: See associated text at Protocol Exchange: Title: “Automated, quantitative Fast FLIM-FRET (qF3): A step-by-step protocol to measure dissociation constants for protein-protein interactions in live-cell screening applications.” LINK doi.org/10.21203/rs.3.pex-1354/v1 And Instructional videos: LINK youtube.com/playlist?list=PLUiSJrzzg9voe5sjA57oIbfOLAGIrHXRc
Links
Where it is published
- Dataverse dataset page borealisdata.ca/dataset.xhtml?persistentId=doi%3A10.5683%2FSP3%2FQYDEDH ↗
landing page · from borealisdata ca
- DOI doi.org/10.5683/sp3/qydedh ↗
DOI / persistent id · from borealisdata ca
Catalogue records · 1
- Dataverse API borealisdata.ca/api/datasets/:persistentId/?persistentId=doi%3A10.5683%2FSP3%2… ↗
metadata API · from borealisdata ca
Topics
Provenance · 2 source records, 21 field assertions
| Source | Key | Last seen | Raw |
|---|---|---|---|
| Borealis | doi:10.5683/SP3/QYDEDH | 13 d ago | JSON v1 |
| Agri-environmental Research Data Dataverse | doi:10.5683/SP3/QYDEDH | 11 d ago | JSON v1 |
| Field | Assertion | Extractor | Evidence |
|---|---|---|---|
| concepts[field].dataverse_subject:other | source · borealisdata ca | connector:borealisdata_ca@1.0.0 | /subjects |
| concepts[field].dataverse_subject:other | source · borealisdata ca dataverse | connector:borealisdata_ca_dataverse@1.0.0 | /subjects |
| concepts[field].local:field:chemistry | mapping · borealisdata ca dataverse | connector:borealisdata_ca_dataverse@1.0.0 | /subjects |
| concepts[field].local:field:computer-science-ai | mapping · borealisdata ca dataverse | connector:borealisdata_ca_dataverse@1.0.0 | /subjects |
| concepts[field].local:field:earth-environmental | mapping · borealisdata ca dataverse | connector:borealisdata_ca_dataverse@1.0.0 | /subjects |
| concepts[field].local:field:economics-finance | mapping · borealisdata ca dataverse | connector:borealisdata_ca_dataverse@1.0.0 | /subjects |
| concepts[field].local:field:engineering | mapping · borealisdata ca dataverse | connector:borealisdata_ca_dataverse@1.0.0 | /subjects |
| concepts[field].local:field:humanities | mapping · borealisdata ca dataverse | connector:borealisdata_ca_dataverse@1.0.0 | /subjects |
| concepts[field].local:field:life-sciences | mapping · borealisdata ca dataverse | connector:borealisdata_ca_dataverse@1.0.0 | /subjects |
| concepts[field].local:field:mathematics-statistics | mapping · borealisdata ca dataverse | connector:borealisdata_ca_dataverse@1.0.0 | /subjects |
| concepts[field].local:field:medicine-health | mapping · borealisdata ca dataverse | connector:borealisdata_ca_dataverse@1.0.0 | /subjects |
| concepts[field].local:field:ocean-atmospheric | mapping · borealisdata ca dataverse | connector:borealisdata_ca_dataverse@1.0.0 | /subjects |
| concepts[field].local:field:social-science | mapping · borealisdata ca dataverse | connector:borealisdata_ca_dataverse@1.0.0 | /subjects |
| concepts[modality].local:modality:image | enrichment · borealisdata ca | keyword-concept-rules@1.0.0 | title+description (75%) |
| concepts[modality].local:modality:text | enrichment · borealisdata ca | keyword-concept-rules@1.0.0 | title+description (75%) |
| created_date | source · borealisdata ca | connector:borealisdata_ca@1.0.0 | |
| description | source · borealisdata ca | connector:borealisdata_ca@1.0.0 | /description |
| publication_date | source · borealisdata ca | connector:borealisdata_ca@1.0.0 | |
| title | source · borealisdata ca | connector:borealisdata_ca@1.0.0 | /name |
| updated_date | source · borealisdata ca | connector:borealisdata_ca@1.0.0 | |
| version_label | source · borealisdata ca | connector:borealisdata_ca@1.0.0 |