Data · dataset · 2025
ROS-Inducing Micelles Sensitize Tumor-Associated Dendritic cells to TLR3 Stimulation for Potent Immunotherapy
Listed in ScienceDB
Objective To prepare ZnPP-PM nanoparticles based on peptide micelles (PM) loaded with zinc protoporphyrin IX (ZnPP) for targeted delivery of immune adjuvants, and to study their activation ability and anti-tumor effect on tumor associated dendritic cells (TADCs) derived from mouse bone marrow.
Description
Method
ZnPP was connected to mannose functionalized PM block copolymers via amide bonds, self-assembled into ZnPP-PM with the ability to target DC and induce ROS, and loaded with PIC via electrostatic adsorption to form ZnPP-PM/PIC nanoparticles, which were characterized. Application of laser confocal microscopy and flow cytometry to detect the uptake ability of cells towards ZnPP-PM, as well as the effect of ZnPP-PM/PIC on the production and activation of reactive oxygen species (ROS) in TADCs.
Read the rest (1 more)
And verify the in vivo anti-tumor effect of ZnPP-PM/PIC through animal experiments.Result The particle sizes of ZnPP-PM and ZnPP-PM/PIC are approximately 33 ± 3.55 nm and 30 ± 2.47 nm, respectively, with no significant difference between the two. They have good dispersibility and stability, and exhibit specific targeting ability towards DC. In addition, ZnPP-PM/PIC can more effectively promote TADC activation by enhancing ROS production and PIC coordination (P<0.05), and induce more effective anti-tumor effects in vivo by increasing the activation of effector cells (CD8+CD69+, CD4+CD69+ or NK1.1+CD69+) in tumors and TDLNs.Conclusion Based on ZnPP-PM as a targeted transport immune adjuvant, promoting TADC activation can become a potential anti-tumor treatment strategy.
Links
Where it is published
- DOI doi.org/10.57760/sciencedb.j00217.05985 ↗
DOI / persistent id · from scidb cn
Catalogue records · 1
- OAI-PMH record scidb.cn/oai?verb=GetRecord&metadataPrefix=oai_dc&identifier=10.57760%2… ↗
metadata API · from scidb cn
Topics
- From keywords
- Earth & Environmental Science · Engineering · Humanities · Life Sciences · Social Science
- Inferred from text
- Cancer 65% · Microscopy 75%
Provenance · 1 source records, 11 field assertions
| Source | Key | Last seen | Raw |
|---|---|---|---|
| ScienceDB | 10.57760/sciencedb.j00217.05985 | 12 d ago | JSON v1 |
| Field | Assertion | Extractor | Evidence |
|---|---|---|---|
| concepts[disease].local:disease:cancer | enrichment · scidb cn | keyword-concept-rules@1.0.0 | title+description (65%) |
| concepts[field].local:field:earth-environmental | mapping · scidb cn | connector:scidb_cn@1.0.0 | |
| concepts[field].local:field:engineering | mapping · scidb cn | connector:scidb_cn@1.0.0 | |
| concepts[field].local:field:humanities | mapping · scidb cn | connector:scidb_cn@1.0.0 | |
| concepts[field].local:field:life-sciences | mapping · scidb cn | connector:scidb_cn@1.0.0 | |
| concepts[field].local:field:social-science | mapping · scidb cn | connector:scidb_cn@1.0.0 | |
| concepts[modality].local:modality:microscopy | enrichment · scidb cn | keyword-concept-rules@1.0.0 | title+description (75%) |
| description | source · scidb cn | connector:scidb_cn@1.0.0 | /metadata/dc/description |
| license_text | source · scidb cn | connector:scidb_cn@1.0.0 | |
| publication_date | source · scidb cn | connector:scidb_cn@1.0.0 | |
| title | source · scidb cn | connector:scidb_cn@1.0.0 | /metadata/dc/title |