Soraphen A effect on B cells post-stroke

Male Nur77GFP mice (12–14 weeks) underwent tMCAO. At reperfusion, mice received intraperitoneal LPS or vehicle; SorA or vehicle was given 2 h later and daily thereafter. B cell activation and differentiation were analyzed by flow cytometry in lungs, spleen, blood, and lymph nodes at 16 h, 2, 3, and 7 d, with GFP indicating antigen-specific activation.

Results: While both SorA and lipopolysaccharide (LPS) replenished splenic B cell numbers depleted by stroke, their effects on B cell subsets diverge. SorA also reversed LPS-induced reductions in lung B cell counts and decreased plasma cell counts. Both treatments increased B cell activation and modulated MHC-II expression.

In contrast to T cells, the mode of B cell activation was not affected by SorA treatment. Effects of LPS and SorA on B cell populations were most distinct in the lungs. Discussion: These findings demonstrate that the beneficial effects of SorA after stroke are not solely mediated by T cell modulation.

Further investigation into SorA’s organ-specific impact on different B cell subtypes is crucial to fully elucidate its mechanisms of action.