A dataset of VP4 regulates PINK1/Parkin-mediated mitophagy and ubiquitinates p62 for degradation

Subcellular colocalization data: Confocal microscopy images and quantitative analyses showing the dynamic colocalization of VP4 with p62 and Parkin in the cytoplasm over time.4. Gene knockdown and truncation mutant data: Changes in p62 ubiquitination and degradation levels upon Parkin knockdown; effects of VP4 truncation mutants (targeting the PB1 and UBA domains of p62) on mitophagy and viral replication.5. Viral replication indicators: Time‑series measurements of GCRV Ⅱ replication titers, viral protein expression, and cell death levels under different conditions (e.g., VP4 overexpression, autophagy pathway inhibition).Data were collected at weekly or per‑experiment‑batch frequency, with a minimum of three biological replicates per condition.

All raw data have undergone normalization, outlier removal, and standardized archiving. The dataset is formatted for time‑series analysis, differential expression statistics, and pathway enrichment analysis. It is suitable for further studies on how viral proteins hijack host autophagy machinery, modeling virus‑host interactions, and screening anti‑GCRV drug targets.