Omics · study · 2026
Identification of the negamycin biosynthetic genes in Kitasatospora purpeofusca ATCC21470
Listed in NCBI GEO
Negamycin is a ribosome-targeting antibiotic with activity against Gram-positive and Gram-negative ESKAPE pathogens and the ability to promote premature stop codon readthrough.
Description
Its therapeutic potential is limited by low natural production and synthetic complexity. To enable scalable biosynthesis, we identified and characterized its genetic basis in Kitasatospora purpeofusca.
Two distant chromosomal regions, neg1 and neg2, were found to be essential. Deletion of neg1, involved in nitrite formation for N-N bond biosynthesis, reduced production to ~2%, while deletion of neg2, which directs β-lysine formation and scaffold assembly, abolished it completely. Isotope-labeling confirmed nitrite incorporation, and complementation restored production.
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Transcriptomic and proteomic analyses further supported the essential roles of both regions. Expression of neg1 along with the neg2 region in Streptomyces albus reconstituted negamycin biosynthesis. This study reveals an unusual split biosynthetic pathway and provides a foundation for the biotechnological production and further development of this promising antibiotic.
Links
Get the data
- GEO FTP directory ftp.ncbi.nlm.nih.gov/geo/series/GSE304nnn/GSE304071 ↗
download · from NCBI GEO
Where it is published
- GEO accession page ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE304071 ↗
landing page · from NCBI GEO
Documentation and papers
- PRJNA1298865 ncbi.nlm.nih.gov/bioproject/PRJNA1298865 ↗
project · from NCBI GEO
Topics
- Stated by source
- Expression profiling by high throughput sequencing
- From keywords
- Life Sciences
Provenance · 1 source records, 6 field assertions
| Source | Key | Last seen | Raw |
|---|---|---|---|
| NCBI GEO | GSE304071 | 15 d ago | JSON v1 |
| Field | Assertion | Extractor | Evidence |
|---|---|---|---|
| access_level | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[field].local:field:life-sciences | mapping · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[method].geo_series_type:expression-profiling-by-high-throughput-sequencing | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /gdstype |
| description | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /summary |
| publication_date | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| title | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /title |