Allelopathy of Bacillus amyloliquefaciens to Phaeodactylum tricornutum

Count with blood cell counting plate and convert the concentration of long-term culture algal solution. Dilute the algae solution to make its concentration be 20%, 40%, 60%, 80% and 100% of the original respectively.

Measure the absorbance value at 663 nm wavelength, and draw the linear standard curve between algae concentration and absorbance. Inhibition rate formula: PI (%) = (1 － n / N0) × 100%。 Among them, N0 and N are the concentration of Phaeodactylum tricornutum in the control group and the concentration of Phaeodactylum tricornutum in the test group respectively; PI is the inhibition ratio. When pi = 50%, the corresponding concentration is EC50.