Omics · study · 2026
RNA-seq and MeRIP-seq comparison of Mettl3 knockout basal cell derived organoids
Listed in NCBI GEO
Airway basal stem cells are critical for maintaining epithelial homeostasis and repairing tissue damage, yet the epitranscriptomic mechanisms governing their stemness remain poorly understood.
Description
We show that METTL3 deficiency in murine airway basal cells leads to aberrant differentiation, loss of stemness, and impaired organoid formation. RNAseq and MeRIP-seq reveal that METTL3 directly targets the mRNA of Ninj1 for m6A modification, promoting its degradation.
Loss of METTL3 leads to Ninj1 upregulation.
Links
Get the data
- GEO FTP directory ftp.ncbi.nlm.nih.gov/geo/series/GSE303nnn/GSE303982 ↗
download · from NCBI GEO
Where it is published
- GEO accession page ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE303982 ↗
landing page · from NCBI GEO
Documentation and papers
- PRJNA1298357 ncbi.nlm.nih.gov/bioproject/PRJNA1298357 ↗
project · from NCBI GEO
Topics
- Stated by source
- Expression profiling by high throughput sequencing · Mus musculus · Other
- From keywords
- Life Sciences
- Inferred from text
- RNA sequencing 65%
Provenance · 1 source records, 9 field assertions
| Source | Key | Last seen | Raw |
|---|---|---|---|
| NCBI GEO | GSE303982 | 14 d ago | JSON v1 |
| Field | Assertion | Extractor | Evidence |
|---|---|---|---|
| access_level | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[field].local:field:life-sciences | mapping · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[method].geo_series_type:expression-profiling-by-high-throughput-sequencing | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /gdstype |
| concepts[method].geo_series_type:other | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /gdstype |
| concepts[modality].local:modality:rna-seq | enrichment · NCBI GEO | keyword-concept-rules@1.0.0 | title+description (65%) |
| concepts[organism].NCBITaxon:10090 | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /taxon |
| description | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /summary |
| publication_date | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| title | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /title |