Process-dependent chemical fingerprints and acute oral toxicity of four crude extracts of <i>Edgeworthia gardneri</i> (Wall.) Meisn in mice

No mortality or persistent signs of acute toxicity were observed. Body weight increased progressively without sustained suppression attributable to any extract. Organ coefficients and serum biochemical measurements showed sporadic changes without concordant toxicological significance.

In male WDE-treated mice, serum aspartate aminotransferase (AST) increased, whereas alanine aminotransferase (ALT) and alkaline phosphatase (ALP) remained unchanged, and blinded liver scores did not indicate treatment-related injury. Under the tested single-dose conditions, the four <i>E. gardneri</i> flower extracts produced minimal evidence of acute oral toxicity in mice of either sex at 1500 mg/kg extract (crude-herb equivalents: 4.6–13.8 g/kg).

These findings provide a reference for dose selection and methodology in future comparative safety assessments of different extraction processes. Compositional variations among extracts were noted, and their relevance to safety warrants further study.</p>