Data · dataset · 2022
Transcriptome data of Thitarodes xiaojinensis larvae infected with entomopathogenic fungi
Listed in ScienceDB
Sixth-instar Thitarodes xiaojinensis larvae were infected with the blastospores of Ophiocordyceps sinensis (Os), Cordyceps militarist (Cm), or Beauveria bassiana (Bb).
Description
Larvae injected with Ringer’s buffer (RS, 8.05 g NaCl, 0.42 g KCl, and 0.18 g CaCl2 per liter) were used as the control. Each larva was injected with 2 µL of Ringer’s buffer or blastospore suspension in Ringer’s buffer (1.0 × 107 cells/mL) using glass capillaries.
Each treatment included 12 larvae. According to the manufacturer’s instructions, total RNA from the fat body of control and fungus-infected larvae was extracted at 72 hpi using TRIzol reagent (Invitrogen, USA). RNA yields were quantified using a NanoDrop 2000 spectrophotometer.
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RNA integrity was confirmed using the RNA Nano 6000 Assay Kit of the Bioanalyzer 2100 system. A cDNA library was constructed using the total RNA and sequenced with 150 bp paired-end reads on an Illumina NovaSeq platform. Three biological replicates were used for each treatment.
Links
Where it is published
- DOI doi.org/10.11922/sciencedb.o00019.00011 ↗
DOI / persistent id · from scidb cn
Catalogue records · 1
- OAI-PMH record scidb.cn/oai?verb=GetRecord&metadataPrefix=oai_dc&identifier=10.11922%2… ↗
metadata API · from scidb cn
Topics
- From keywords
- Earth & Environmental Science · Engineering · Humanities · Life Sciences · Social Science
Provenance · 1 source records, 9 field assertions
| Source | Key | Last seen | Raw |
|---|---|---|---|
| ScienceDB | 10.11922/sciencedb.o00019.00011 | 12 d ago | JSON v1 |
| Field | Assertion | Extractor | Evidence |
|---|---|---|---|
| concepts[field].local:field:earth-environmental | mapping · scidb cn | connector:scidb_cn@1.0.0 | |
| concepts[field].local:field:engineering | mapping · scidb cn | connector:scidb_cn@1.0.0 | |
| concepts[field].local:field:humanities | mapping · scidb cn | connector:scidb_cn@1.0.0 | |
| concepts[field].local:field:life-sciences | mapping · scidb cn | connector:scidb_cn@1.0.0 | |
| concepts[field].local:field:social-science | mapping · scidb cn | connector:scidb_cn@1.0.0 | |
| description | source · scidb cn | connector:scidb_cn@1.0.0 | /metadata/dc/description |
| license_text | source · scidb cn | connector:scidb_cn@1.0.0 | |
| publication_date | source · scidb cn | connector:scidb_cn@1.0.0 | |
| title | source · scidb cn | connector:scidb_cn@1.0.0 | /metadata/dc/title |