Imaging · dataset · 2026
IBEX immunofluorescence images of skin from CD11c-DOG mice treated or untreated with diphtheria toxin
Listed in Bicocca Open Archive Research Data
Iterative Bleaching Extends Multiplexity (IBEX) immunofluorescence was performed on skin samples collected from CD11c-DOG mice either treated with diphtheria toxin (Skin_CD11cDOG_with_Diphtheria_Toxin) or phosphate-buffered saline (PBS) treated controls (Skin_CD11cDOG_no_Diphtheria_Toxin).
Description
For dendritic cell depletion, CD11c-DOG mice received three systemic intravenous injections of diphtheria toxin (16 ng/g body weight) on days −3, −2, and −1.
Control mice PBS following the same schedule. In addition, a local subcutaneous injection of diphtheria toxin (16 ng) was administered on day −1 at the site designated for tissue collection. Mice were euthanized on day 0 in accordance with national and international regulations for animal care and use.
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A square section of flank skin was excised from each mouse at the site of the last intradermal injection. Subcutaneous adipose tissue was mechanically removed using forceps. Skin samples were subsequently processed according to the 2D Manual IBEX protocol for multiplex immunofluorescence imaging.
Links
Where it is published
- DOI doi.org/10.17632/sytvr3j78g.1 ↗
DOI / persistent id · from board unimib it
Catalogue records · 1
- OAI-PMH record board.unimib.it/oai?verb=GetRecord&metadataPrefix=oai_dc&identifier=oai%3Aboar… ↗
metadata API · from board unimib it
Topics
- From keywords
- Earth & Environmental Science · Engineering · Humanities · Life Sciences · Microscopy · Social Science
Provenance · 1 source records, 12 field assertions
| Source | Key | Last seen | Raw |
|---|---|---|---|
| Bicocca Open Archive Research Data | oai:board.unimib.it/sytvr3j78g.1 | 5 d ago | JSON v1 |
| Field | Assertion | Extractor | Evidence |
|---|---|---|---|
| concepts[field].local:field:earth-environmental | mapping · board unimib it | connector:board_unimib_it@1.0.0 | |
| concepts[field].local:field:engineering | mapping · board unimib it | connector:board_unimib_it@1.0.0 | |
| concepts[field].local:field:humanities | mapping · board unimib it | connector:board_unimib_it@1.0.0 | |
| concepts[field].local:field:life-sciences | mapping · board unimib it | connector:board_unimib_it@1.0.0 | |
| concepts[field].local:field:social-science | mapping · board unimib it | connector:board_unimib_it@1.0.0 | |
| concepts[modality].local:modality:image | enrichment · board unimib it | keyword-concept-rules@1.0.0 | title+description (65%) |
| concepts[modality].local:modality:imaging | enrichment · board unimib it | keyword-concept-rules@1.0.0 | title+description (75%) |
| concepts[modality].local:modality:microscopy | mapping · board unimib it | vocabulary-mapper@1.0.0 | keywords['Fluorescence Microscopy'] |
| description | source · board unimib it | connector:board_unimib_it@1.0.0 | /metadata/dc/description |
| license_text | source · board unimib it | connector:board_unimib_it@1.0.0 | |
| publication_date | source · board unimib it | connector:board_unimib_it@1.0.0 | |
| title | source · board unimib it | connector:board_unimib_it@1.0.0 | /metadata/dc/title |