Data · dataset
Screening Antimicrobial Activity of Actinomycetes Isolated from Raja Ampat, West Papua, Indonesia
Listed in RIN Data Repository
<p> In the framework of exploitation of antimicrobial activity of Actinomycetes in Papua, one hundred isolates of Actinomycetes isolated from soil and leaf litter samples from various ecosystems in Batanta and Salawati Island, Raja Ampat, West Papua were screened.
Description
We obtained 200 crude extracts from 100 isolates based on two extraction phases. Nonpolar metabolites were extracted by ethyl acetate : methanol (4:1) solvent while the polar metabolites were concentrated using a freeze-drying method.
Based on the agar dilution method, a total of 43 from 200(21.5%) crude extracts have antimicrobial activity against bacteria and yeasts (Escherichia coli NBRC 14237, Bacillus subtilis NBRC 3134, Staphylococcus aureus NBRC 13276, Micrococcus luteus NBRC 1367, Candida albicans NBRC 1594 and Saccharomyces cerevisiae NBRC 10217). Some crude extracts showed anti-Gram negative (1.5%), anti-Gram positive (17%) and antifungal (17%) activities.
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Crude metabolites which were extracted using ethyl acetate : methanol were more effective on antimicrobial activity (35%) compared with water extraction (17%). Five most potential isolates (BL- 13-5, BL-06-5, BL-14-2, BL-22-3, and Sl-36-1) were identified based on 16S rRNA gene sequence data. Sequence similarity search by BLAST program revealed that they show sequence similarities to Streptomyces kanamyceticus (92%), Streptomyces verne (92%), Streptomyces narbonensis (92%), Streptomyces malachitofuscus (98%), and Streptomyces hygroscopicus (96%), respectively. </p> <p> Makara Journal of Science, Vol. 16, No. 1.
Hal. 21-26 </p>
Links
Where it is published
- RIN Data Repository record hdl.handle.net/20.500.12690/RIN/2Y2JSW ↗
landing page · from rin brin go id
Catalogue records · 1
- OAI-PMH record data.brin.go.id/oai?verb=GetRecord&metadataPrefix=oai_dc&identifier=hdl%3A20.5… ↗
metadata API · from rin brin go id
Topics
- From keywords
- Chemistry · Earth & Environmental Science
Provenance · 1 source records, 4 field assertions
| Source | Key | Last seen | Raw |
|---|---|---|---|
| RIN Data Repository | hdl:20.500.12690/RIN/2Y2JSW | 9 d ago | JSON v1 |
| Field | Assertion | Extractor | Evidence |
|---|---|---|---|
| concepts[field].local:field:chemistry | mapping · rin brin go id | connector:rin_brin_go_id@1.0.0 | |
| concepts[field].local:field:earth-environmental | mapping · rin brin go id | connector:rin_brin_go_id@1.0.0 | |
| description | source · rin brin go id | connector:rin_brin_go_id@1.0.0 | /metadata/dc/description |
| title | source · rin brin go id | connector:rin_brin_go_id@1.0.0 | /metadata/dc/title |