Collaborative research: Antarctic diatom proteorhodopsins: Characterization and a potential role in the iron-limitation response

Our research objectives will be achieved through a combination of molecular, biochemical and physiological measurements in diatom isolates recently obtained from the Western Antarctic Peninsula region. We will determine the proton-pumping characteristics and pumping rates of PR as a function of light intensity and wavelength, the resultant PR-linked intracellular ATP production rates, and the cellular localization of the protein.

We will examine under which environmental conditions Antarctic diatom-PR is most highly expressed and construct a cellular energy budget that includes diatom-PR when grown under these different growth conditions. Estimates of the energy flux generated by PR in PR-containing diatoms will be compared to total energy generation by the photosynthetic light reactions and metabolically coupled respiration rates. Finally, we will compare the characteristics and gene expression of diatom-PR in Antarctic diatoms to PR-containing diatoms isolated from temperate regions in order to investigate if there is a preferential dependence on energy production through diatom-PR in diatoms residing in cold, iron-limited regions of the ocean.