Data · dataset · 2026
<p>Analysis of mETC complexes and ISPR.</p>
Listed in ZivaHub
<p>(A) Heatmaps of the complexome profiles from whole-cell complexome of mETC complex subunits from complex II, complex III and complex IV.
Description
Each row represents a gel slice, with the gel slice number indicated on the left and the molecular weight, based on the migration of soluble proteins (blue), on the right. Subunit IDs are shown at the bottom of their corresponding profile, with mitochondrially-encoded subunits highlighted in red.
Dark blue indicates the highest relative abundance (1) and white the lowest (0). Coomassie-stained Native PAGE gel on which the analysis was performed is depicted on the left of the profile. (B) Re-analysis of <i>Toxoplasma</i> complex II immunoprecipitation from [<a href="plospathogens.org/article/info:doi/10.1371/journal.ppat.1014543#ppat.1014543.ref013" target="_blank">13</a>], with putative <i>Tg</i>SDHC added to the search database.
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Confirmed <i>Toxoplasma</i> complex II subunits highlighted in blue and putative <i>Tg</i>SHDC highlighted in purple. (C) Alignment of ISPR proteins from <i>Perkinsus olensi (</i>ISPR1, A0A7J6TKI1; ISPR2, A0A7J6PI35), <i>Toxoplasma gondii</i> (S7UWH3), <i>Besnoitia besnoiti</i> (A0A2A9M9Y8), <i>Cystoisospora suis</i> (A0A2C6LGZ5), <i>Eimeria brunetti</i> (U6LJF9) and <i>Cyclospora cayetanensis</i> (A0A1D3D252). Alignments were made using Clustal Omega and visualised using JalView (v10.0.7) [<a href="plospathogens.org/article/info:doi/10.1371/journal.ppat.1014543#ppat.1014543.ref099" target="_blank">99</a>].
(D) Structural predictions, generated using Alphafold3 [<a href="plospathogens.org/article/info:doi/10.1371/journal.ppat.1014543#ppat.1014543.ref100" target="_blank">100</a>], of <i>P. olensi</i> ISPR1 (pTM score 0.65) (left) and <i>T. gondii</i> ISPR (pTM score 0.69) (middle), coloured according to pIDDT score, as well as superimposition of the two structures (right; <i>PoI</i>SPR1 in blue<i>, Tg</i>ISPR in purple).
(RMSD = 0.73 Å between pruned atom pairs). Visualised using ChimeraX [<a href="plospathogens.org/article/info:doi/10.1371/journal.ppat.1014543#ppat.1014543.ref092" target="_blank">92</a>]. (E) Transcript levels of TGME49_224932 in tachyzoites and bradyzoites from [<a href="plospathogens.org/article/info:doi/10.1371/journal.ppat.1014543#ppat.1014543.ref042" target="_blank">42</a>] and [<a href="plospathogens.org/article/info:doi/10.1371/journal.ppat.1014543#ppat.1014543.ref043" target="_blank">43</a>].
(F) Immunoblot analysis of <i>Toxoplasma gondii</i> expressing a Ty-epitope tagged version of <i>Tg</i>ISPR using the anti-Ty antibody.</p> <p>(TIF)</p>
Links
Where it is published
- DOI doi.org/10.1371/journal.ppat.1014543.s002 ↗
DOI / persistent id · from zivahub uct ac za
Catalogue records · 1
- OAI-PMH record api.figshare.com/v2/oai?verb=GetRecord&metadataPrefix=oai_dc&identifier=oai%3Af… ↗
metadata API · from zivahub uct ac za
Topics
- From keywords
- Biochemistry and cell biology · Earth & Environmental Science · Evolutionary biology · Genetics · Life Sciences · Microbiology
Provenance · 1 source records, 11 field assertions
| Source | Key | Last seen | Raw |
|---|---|---|---|
| ZivaHub | oai:figshare.com:article/34025419 | 13 d ago | JSON v1 |
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| access_level | source · zivahub uct ac za | connector:zivahub_uct_ac_za@1.0.0 | |
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| concepts[field].anzsrc:group:3104 | mapping · zivahub uct ac za | vocabulary-mapper@1.0.0 | keywords['Evolutionary Biology'] |
| concepts[field].anzsrc:group:3105 | mapping · zivahub uct ac za | vocabulary-mapper@1.0.0 | keywords['Genetics'] |
| concepts[field].anzsrc:group:3107 | mapping · zivahub uct ac za | vocabulary-mapper@1.0.0 | keywords['Microbiology'] |
| concepts[field].local:field:earth-environmental | mapping · zivahub uct ac za | connector:zivahub_uct_ac_za@1.0.0 | |
| concepts[field].local:field:life-sciences | mapping · zivahub uct ac za | connector:zivahub_uct_ac_za@1.0.0 | |
| description | source · zivahub uct ac za | connector:zivahub_uct_ac_za@1.0.0 | /metadata/dc/description |
| license | source · zivahub uct ac za | connector:zivahub_uct_ac_za@1.0.0 | /metadata/dc/rights |
| publication_date | source · zivahub uct ac za | connector:zivahub_uct_ac_za@1.0.0 | |
| title | source · zivahub uct ac za | connector:zivahub_uct_ac_za@1.0.0 | /metadata/dc/title |