Omics · study · 2026
Cross-species single cell transcriptomics in fly and beetle reveals the genetic core of brain neuroblast specification
Listed in NCBI GEO
This work defines the conserved core of TFs involved in insect brain NB specification by cross-species comparison of single-cell transcriptomics of NBs from, the red flour beetle, Tribolium castaneum, and the vinegar fly, Drosophila melanogaster.
Description
In Tribolium, we established a Gal4 enhancer trap screen system to generate a line that marks NBs. Using the 10x genomics technology, we sequenced 37,137 Tribolium NBs.
We also sequenced 32,112 single nuclei of Drosophila NBs. Cross-species comparison followed by in situ hybridization led to the identification brain-specific TFs of insects. This brain NB-specific gene set confirms fundamentally different spatial patterning compared to the well-studied ventral nerve cord.
Links
Get the data
- GEO FTP directory ftp.ncbi.nlm.nih.gov/geo/series/GSE337nnn/GSE337265 ↗
download · from NCBI GEO
Where it is published
- GEO accession page ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE337265 ↗
landing page · from NCBI GEO
Documentation and papers
- PRJNA1484590 ncbi.nlm.nih.gov/bioproject/PRJNA1484590 ↗
project · from NCBI GEO
Topics
- Stated by source
- Expression profiling by high throughput sequencing
- From keywords
- Life Sciences
- Inferred from text
- Evolutionary biology 72%
Provenance · 1 source records, 7 field assertions
| Source | Key | Last seen | Raw |
|---|---|---|---|
| NCBI GEO | GSE337265 | 15 d ago | JSON v1 |
| Field | Assertion | Extractor | Evidence |
|---|---|---|---|
| access_level | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[field].anzsrc:group:3104 | enrichment · NCBI GEO | taxonomy-embedding@1.1.0 | title+keywords+description (72%) |
| concepts[field].local:field:life-sciences | mapping · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[method].geo_series_type:expression-profiling-by-high-throughput-sequencing | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /gdstype |
| description | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /summary |
| publication_date | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| title | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /title |