Data · study · 2026
SAM68 is a crucial post-transcriptional regulator of cardiogenesis
Listed in NCBI GEO
RNA-binding proteins (RBPs) of the STAR family play important roles in mammalian development, yet their precise contributions to lineage specification remain incompletely understood.
Description
Here, using CRISPR–Cas9 knockout models combined with multi-omics approaches, we investigate the roles of two STAR proteins, SAM68 and QKI, in mouse embryonic stem cells (mESCs). Both RBPs contribute to mESC proliferation, self-renewal, and efficient differentiation into cardiomyocytes.
Our data support a role for QKI in the regulation of cardiac differentiation programs. In addition, our analyses reveal a previously underappreciated contribution of SAM68 to cardiomyocyte specification, acting through multiple post-transcriptional mechanisms. Despite belonging to the same protein family, SAM68 and QKI regulate largely distinct post-transcriptional programs during differentiation.
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Specifically, SAM68 modulates alternative splicing and promotes the biogenesis of a subset of cardiac-enriched circular RNAs through binding to intronic regions flanking back-splice sites and association with NF90/110. In addition, SAM68 associates with untranslated regions of key differentiation-related transcripts, including Gata4 mRNA, a key transcription factor regulating cardiogenesis, and likely functions in complex with other RNA-binding proteins to regulate these transcripts.
Together, these findings identify SAM68 as a regulator of multiple RNA processing pathways contributing to cardiomyocyte differentiation and provide insight into how STAR proteins shape post-transcriptional gene regulatory networks during early development.
Links
Get the data
- GEO FTP directory ftp.ncbi.nlm.nih.gov/geo/series/GSE326nnn/GSE326251 ↗
download · from NCBI GEO
Where it is published
- GEO accession page ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE326251 ↗
landing page · from NCBI GEO
Documentation and papers
- PRJNA1445016 ncbi.nlm.nih.gov/bioproject/PRJNA1445016 ↗
project · from NCBI GEO
- PubMed 42240622 pubmed.ncbi.nlm.nih.gov/42240622 ↗
publication · from NCBI GEO
Topics
- Stated by source
- Mus musculus · Other
- From keywords
- Life Sciences
Provenance · 1 source records, 9 field assertions
| Source | Key | Last seen | Raw |
|---|---|---|---|
| NCBI GEO | GSE326251 | 15 d ago | JSON v1 |
| Field | Assertion | Extractor | Evidence |
|---|---|---|---|
| access_level | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[anatomy].local:anatomy:heart | enrichment · NCBI GEO | keyword-concept-rules@1.0.0 | title+description (65%) |
| concepts[field].anzsrc:group:3105 | enrichment · NCBI GEO | taxonomy-embedding@1.1.0 | title+keywords+description (68%) |
| concepts[field].local:field:life-sciences | mapping · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[method].geo_series_type:other | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /gdstype |
| concepts[organism].NCBITaxon:10090 | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /taxon |
| description | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /summary |
| publication_date | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| title | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /title |