Data Sheet 1_A broad-host-range inhalable phage cocktail for treatment of multidrug-resistant Pseudomonas aeruginosa pulmonary infections.pdf

The limited efficacy of traditional antibiotics against this pathogen highlights the necessity of developing alternative treatments. Our study aims to develop a broad-spectrum bacteriophage cocktail suitable for nebulization and to evaluate its antibacterial efficacy and safety in respiratory infections caused by MDR P. aeruginosa.</p>Methods<p>We constructed a seven-phage cocktail by selecting phages with complementary host ranges, strong lytic activity, and different receptor targets.

The antimicrobial and anti-biofilm activity of the cocktail was assessed in vitro against clinical MDR P. aeruginosa isolates. The therapeutic efficacy and safety were further investigated in a murine pneumonia model following nebulized administration.</p>Results<p>The phage cocktail demonstrated lytic activity against nearly 90% of 152 clinical P. aeruginosa isolates, indicating broad coverage. Compared to single phages or antibiotics, the cocktail showed superior activity in both inhibiting and disrupting biofilms.