Table · dataset · 2026
Eugenol-Functionalized Bovine Bone Substitutes for Potential Osteogenic Applications
Listed in ZivaHub and Deakin Research Online — shown once because both records carry DOI 10.1021/acsomega.6c06432.s001
The study explored the role of eugenol-functionalized bovine bone substitutes in mitigating oxidative stress and promoting osteogenesis in vitro.
Description
Bovine bones were subjected to decellularization employing physicochemical and enzymatic procedures to prepare decellularized bovine bone scaffolds (DCC). Eugenol-chitosan functionalized DCC (ECF-DCC) bone substitutes were fabricated by ball-milling DCC scaffolds, spray-drying eugenol-chitosan particulates, integrating β-TCP, and lyophilizing the homogenized composites.
ECF-DCC were comprehensively assessed by SEM-EDS, RAMAN spectroscopy, and osteoblast-based viability, apoptosis, reactive oxygen species (ROS) generation, osteogenic differentiation [alkaline phosphatase (ALP), mineralization], real-time PCR, and Western blotting. RAMAN spectroscopy confirmed successful eugenol functionalization of DCC substitutes, evidenced by characteristic aromatic CC and C–O vibrational peaks alongside preserved phosphate signatures.
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SEM–EDS revealed rough substitutes in 500–750 μm size with comparable elemental profiles. hFOB 1.19 cells were viable across all concentrations, with no significant increase in apoptosis, although a low-level necrosis was observed at 1000 μg/mL. ECF-DCC significantly reduced ROS generation compared to DCC. Gene expression analysis showed that ECF-DCC markedly reduced key proinflammatory cytokines including <i>IL</i>-<i>6</i>, <i>IL</i>-<i>1β</i>, and <i>TNF</i>-<i>α</i>.
Both formulations enhanced osteogenic differentiation, with ECF-DCC inducing superior upregulation of RUNX2, OC, Col1, and BMP-7 at mRNA and protein levels. ECF-DCC substitutes also showed highest ALP activity and calcium mineralization, confirming their enhanced osteoinductive potential. Eugenol-chitosan functionalization enhanced the biological performance of DCC substitutes by reducing oxidative stress and inflammatory responses while significantly promoting osteogenic differentiation, highlighting potential use as improved osteoinductive bone substitutes.
Links
Where it is published
- DOI doi.org/10.1021/acsomega.6c06432.s001 ↗
DOI / persistent id · from zivahub uct ac za
Catalogue records · 1
- OAI-PMH record api.figshare.com/v2/oai?verb=GetRecord&metadataPrefix=oai_dc&identifier=oai%3Af… ↗
metadata API · from zivahub uct ac za
Topics
- From keywords
- Astronomy & Astrophysics · Astronomy & Astrophysics · Biochemistry and cell biology · Biochemistry and cell biology · Cancer · Cancer · Chemistry · Chemistry · Earth & Environmental Science · Earth & Environmental Science · Ecology · Ecology · Life Sciences · Life Sciences · Medicine & Health · Medicine & Health
Provenance · 2 source records, 21 field assertions
| Source | Key | Last seen | Raw |
|---|---|---|---|
| ZivaHub | oai:figshare.com:article/34030488 | 13 d ago | JSON v1 |
| Deakin Research Online | oai:figshare.com:article/34030488 | 13 d ago | JSON v1 |
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|---|---|---|---|
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| concepts[field].anzsrc:group:3101 | mapping · zivahub uct ac za | vocabulary-mapper@1.0.0 | keywords['Cell Biology'] |
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| title | source · zivahub uct ac za | connector:zivahub_uct_ac_za@1.0.0 | /metadata/dc/title |