The discovery and evaluation of a novel bioactive peptide, QUB-3112, from the defensive skin secretion of Hylarana guentheri

They are a rich source of bioactive compounds, including bufogenins, steroids, peptides, etc. To date, many peptides with various bioactivities have been found in frog skin secretions.

Among these, the majority are antimicrobial peptides and a novel one of these was studied in this thesis in order to contribute to the study of biologically active peptides.<br>In this study, the precursor-encoding cDNA sequence of a novel bioactive peptide QUB-3112 was identified in the defensive skin secretion of Hylarana guentheri by using a ‘shotgun’ cloning method. To research the biological functions of QUB-3112, it was synthesised by using solid phase peptide synthesis (SPPS) according to the translated putative mature peptide sequence.