Metabolomic analysis of Liriomyza trifolii in response to two host plants, sponge gourd and kidney bean

The mobile phases were 0.1% formic acid in water (A) and 0.1% formic acid in acetonitrile (B) for both positive and negative ion modes, with an injection volume of 2 μL. Under the control of MassLynx V4.2 software, the mass spectrometer operated in MSe mode to acquire both MS and MS/MS data within each cycle, simultaneously performing low-energy (off) and high-energy (10-40 V) scans at a frequency of 0.2 s per spectrum.

ESI source parameters were set as follows: capillary voltage 2500 V (positive) or -2000 V (negative), cone voltage 30 V, ion source temperature 100°C, desolvation temperature 500°C, cone gas flow 50 L/h, and desolvation gas flow 800 L/h. Raw data were processed with Progenesis QI software for peak picking, alignment, and compound identification against the METLIN online database and an in-house library. After total peak area normalization, principal component analysis and Spearman correlation analysis were used to assess sample repeatability and quality control performance.