A temporal factorial dissection of kidney organoid differentiation identifies the impact of WNT, FGF, BMP and GDF signaling on ‘off-target’ patterning

Assessing how accurately protocols recapitulate development is challenged by the lack of accurate reference data sets for early human development, the presence of ‘off target’ states and the potential to form cellular states not presentin vivo. This study addresses these challenges with respect to differentiation of human pluripotent stem cells to kidney organoids.

Based on a factorial single-cell transcriptomic analysis across a 27-day differentiation protocol, including 150,957 cells collected at five time points, we present a comprehensive classification of predicted identity, representing anin vitrotemporal transcriptional atlas. A significant component of all timepoints were neuromesodermal ‘off target’ populations which resulted in a variety of neural/neural crest and muscle endpoints.