Omics · study · 2026
LuxR regulates Flavobacterium covae pathogenesis in channel catfish (Ictalurus punctatus)
Listed in NCBI GEO
Columnaris disease, caused by Flavobacterium covae, is one of three primary bacterial diseases that cause significant mortality and economic losses in farmed catfish (Ictalurus spp.).
Description
The quorum sensing gene luxR was reported to be upregulated in F. covae biofilm-grown cells stimulated with channel catfish (I. punctatus) mucus. To understand LuxR’s contributions to F. covae virulence, a F. covae C#2 luxR deletion mutant (ΔluxR) was created along with a complemented luxR (ΔluxR + pCE1).
Channel catfish fingerlings challenged with the wild type or ΔluxR + pCE1 were observed to have 100% or 90% mortality, respectively; in contrast, only 2% mortality was observed for catfish fingerlings challenged with ΔluxR, indicating the important role for LuxR-regulated genes in F. covae virulence. A transcriptomic analysis indicated that LuxR-regulated genes in F. covae encode many functions including those associated with virulence, bacterial growth and metabolism, and survival during a fish infection.
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A decrease in biofilm formation was observed for ΔluxR relative to the wild-type and the complemented mutant, but only when mannose was included in the growth medium. The results of this study indicated that LuxR is a critical regulator of F. covae quorum sensing and virulence, warranting further research to identify and describe the relative contributions of LuxR-regulated virulence factors.
Links
Get the data
- GEO FTP directory ftp.ncbi.nlm.nih.gov/geo/series/GSE328nnn/GSE328594 ↗
download · from NCBI GEO
Where it is published
- GEO accession page ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE328594 ↗
landing page · from NCBI GEO
Documentation and papers
- PRJNA1442102 ncbi.nlm.nih.gov/bioproject/PRJNA1442102 ↗
project · from NCBI GEO
Topics
- Stated by source
- Expression profiling by high throughput sequencing
- From keywords
- Life Sciences
- Inferred from text
- Disease 75%
Provenance · 1 source records, 7 field assertions
| Source | Key | Last seen | Raw |
|---|---|---|---|
| NCBI GEO | GSE328594 | 13 d ago | JSON v1 |
| Field | Assertion | Extractor | Evidence |
|---|---|---|---|
| access_level | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[disease].local:disease:disease | enrichment · NCBI GEO | keyword-concept-rules@1.0.0 | title+description (75%) |
| concepts[field].local:field:life-sciences | mapping · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[method].geo_series_type:expression-profiling-by-high-throughput-sequencing | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /gdstype |
| description | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /summary |
| publication_date | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| title | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /title |