Dynamic immune regulation of Gymnocypris eckloni in response to severe short-term hypoxia and moderate prolonged hypoxia

As a typical endemic fish species inhabiting the Qinghai-Tibetan Plateau, Gymnocypris eckloni displays outstanding resilience to hypoxia, however, limited knowledge exists regarding the dynamic changes of immune system in G. eckloni to hypoxic environments with varying DO levels. In this study, G. eckloni liver and blood biochemical parameters and transcriptomes were investigated in response to severe hypoxia for 12 h and moderate hypoxia for different durations (24, 96 and 168 h) to highlight the differences in immune responses, and the expression patterns of key immune-related genes in the gills and spleen following hypoxia stress were also analyzed.

Integrating biochemical parameters and mRNA expression profiles, we found that severe short-term hypoxia exacerbated oxidative damage and significantly altered the expression of genes related to apoptosis and autophagy compared with moderate prolonged hypoxia. Phagosome, lysosome, TLR and NLR signaling pathways were significantly enriched under severe short-term hypoxia, whereas no comparable enrichment was observed under moderate prolonged hypoxia.