Data for Site-specific conjugation of antifreeze proteins onto polymer-stabilized nanoparticles

Here, a range of engineered AFP mutants were prepared and site-specifically conjugated onto RAFT polymer-stabilized gold nanoparticles to generate new hybrid multivalent ice growth inhibitors. Only the SNAP-tagged AFPs lead to potent ‘antifreeze’ active nanomaterials with His-Tag capture resulting in no activity, showing the mode of conjugation is essential.

This versatile strategy will enable the development of multivalent AFPs for translational and fundamental studies.<br><br>Background data is contained in Excel or original format. This dataset contains: - Nanoparticle and polymer characterisation data (DLS, SEC) - Ice growth data from the ‘splat’ assay