Data · dataset · 2026
<p>EGL-2(G574E) depends on the potassium selectivity filter and the intrinsic ligand.</p>
Listed in UCL Research Data Repository
<p>(A) Conservation of the potassium selectivity filter in EAG channels.
Description
Top panel: amino acid sequence alignment of the selectivity filter region of rEAG1, dEAG, and EGL-2. The red arrowhead marks the conserved phenylalanine (F) residue; its substitution with alanine (F456A) in dEAG abolishes potassium currents.
Bottom panel: side views of the selectivity filter formed by two adjacent subunits of rEAG1 (PDB:5K7L) or EGL-2 (predicted by AlphaFold3). (B) Structural model of the PAS-CNBHD interaction in EGL-2, predicted by AlphaFold3. The PAS domain and CNBHD shown are from adjacent subunits.
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The intrinsic ligand motif (Y686N687L688), located near the end of CNBHD, is important for PAS-CNBHD interaction. (<b>C-D</b>) Quantification of sleep behavior shown as the fraction of quiescent worms for the indicated genotypes and conditions. All strains contain the transgene (HS::EGF) for conditional overexpression of EGF via 15-min heat shock (HS) at 33 °C to induce sleep.
Pre and post refer to before and 2 h after heat shock, respectively. (C) EGL-2 (F452A), the equivalent of the dEAG (F456A) mutation that abolishes potassium currents, was introduced into the endogenous <i>egl-2(tan200[G574E])</i> locus. (D) The intrinsic ligand motif (Y686N687L688) of EGL-2 was mutated to ANA in the endogenous <i>egl-2(tan200[G574E])</i> locus.
Each dot represents one independent assay containing 15–25 animals on a single plate. Mean ± SEM are shown. ****, p < 0.0001. One-way ANOVA with Dunnett’s multiple-comparisons test for post-heat shock samples, comparing each genotype to <i>egl-2(G574E)</i> mutants.</p>
Links
Where it is published
- DOI doi.org/10.1371/journal.pgen.1012255.g005 ↗
DOI / persistent id · from rdr ucl ac uk
Catalogue records · 1
- OAI-PMH record api.figshare.com/v2/oai?verb=GetRecord&metadataPrefix=oai_dc&identifier=oai%3Af… ↗
metadata API · from rdr ucl ac uk
Topics
- From keywords
- Biochemistry and cell biology · Caenorhabditis elegans · Cancer · Earth & Environmental Science · Genetics · Life Sciences · Medicine & Health
Provenance · 1 source records, 12 field assertions
| Source | Key | Last seen | Raw |
|---|---|---|---|
| UCL Research Data Repository | oai:figshare.com:article/34049536 | 13 d ago | JSON v1 |
| Field | Assertion | Extractor | Evidence |
|---|---|---|---|
| access_level | source · rdr ucl ac uk | connector:rdr_ucl_ac_uk@1.0.0 | |
| concepts[disease].local:disease:cancer | mapping · rdr ucl ac uk | vocabulary-mapper@1.0.0 | keywords['Cancer'] |
| concepts[field].anzsrc:group:3101 | mapping · rdr ucl ac uk | vocabulary-mapper@1.0.0 | keywords['Cell Biology'] |
| concepts[field].anzsrc:group:3105 | mapping · rdr ucl ac uk | vocabulary-mapper@1.0.0 | keywords['Genetics'] |
| concepts[field].local:field:earth-environmental | mapping · rdr ucl ac uk | connector:rdr_ucl_ac_uk@1.0.0 | |
| concepts[field].local:field:life-sciences | mapping · rdr ucl ac uk | connector:rdr_ucl_ac_uk@1.0.0 | |
| concepts[field].local:field:medicine-health | mapping · rdr ucl ac uk | connector:rdr_ucl_ac_uk@1.0.0 | |
| concepts[organism].NCBITaxon:6239 | mapping · rdr ucl ac uk | vocabulary-mapper@1.0.0 | keywords['caenorhabditis elegans </'] |
| description | source · rdr ucl ac uk | connector:rdr_ucl_ac_uk@1.0.0 | /metadata/dc/description |
| license | source · rdr ucl ac uk | connector:rdr_ucl_ac_uk@1.0.0 | /metadata/dc/rights |
| publication_date | source · rdr ucl ac uk | connector:rdr_ucl_ac_uk@1.0.0 | |
| title | source · rdr ucl ac uk | connector:rdr_ucl_ac_uk@1.0.0 | /metadata/dc/title |