Omics · study · 2026
Intestine epithelial Ceacam1 deficiency prevents steroid-refractory acute gut graft-versus-host disease
Listed in NCBI GEO
Steroid refractory gut acute graft-versus-host disease (SR-Gut-aGVHD) is the major cause of non-relapse death after allogeneic hematopoietic cell transplantation, due to poor understanding its pathogenesis.
Description
We recently reported that increase of donor-type IL-22+ T cells, IL-22-dependent dysbiosis, and loss of anti-inflammatory CX3CR1hi mononuclear phagocytes (MNP) play critical roles in SR-Gut-aGVHD pathogenesis, but the mechanisms remain unclear.
Ceacam1 on intestinal epithelial cells (IECs) is proposed to regulate bacterial translocation and subsequent immune response in the gut tissues. Here, with imaging mass cytomtory (IMC), combined scRNA-Seq and ATAC-Seq, as well as high dimensional flow cytometry analysis, we show that Ceacam1 expression is enhanced on IECs in murine and human SR-Gut-aGVHD. Ceacam1 deficiency on host IECs effectively prevents SR-Gut-aGVHD in murine models.
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Ceacam1 deficiency on IECs results in 1) increase in frequencies of IL-22+IL-10+Foxp3+CD4+ peripheral Tregs (pTregs) while reducing conventional IL-22+CD4+ T (Tcon), Th/Tc1, and Th17 cells; 2) increase of beneficial commensal bacterial that augment colonic pTregs expansion, while reducing pathogenic bacteria and blocking E. Coli’s interaction with IECs; and 3) increase of anti-inflammatory CD103-CX3CR1hi MNP that produce IDO and IL-10, while reducing pro-inflammatory CD103+CX3CR1lo MNP that produce IL-6.
Thus, specific targeting IEC Ceacam1 represents a novel approach for prevention of SR-Gut-aGVHD.
Links
Get the data
- GEO FTP directory ftp.ncbi.nlm.nih.gov/geo/series/GSE268nnn/GSE268993 ↗
download · from NCBI GEO
Where it is published
- GEO accession page ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE268993 ↗
landing page · from NCBI GEO
Documentation and papers
- PRJNA1119637 ncbi.nlm.nih.gov/bioproject/PRJNA1119637 ↗
project · from NCBI GEO
Topics
- Stated by source
- Expression profiling by high throughput sequencing · Genome binding/occupancy profiling by high throughput sequencing · Mus musculus
- From keywords
- Life Sciences
- Inferred from text
- Disease 75% · Imaging 75% · Single-cell RNA sequencing 65%
Provenance · 1 source records, 11 field assertions
| Source | Key | Last seen | Raw |
|---|---|---|---|
| NCBI GEO | GSE268993 | 15 d ago | JSON v1 |
| Field | Assertion | Extractor | Evidence |
|---|---|---|---|
| access_level | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[disease].local:disease:disease | enrichment · NCBI GEO | keyword-concept-rules@1.0.0 | title+description (75%) |
| concepts[field].local:field:life-sciences | mapping · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[method].geo_series_type:expression-profiling-by-high-throughput-sequencing | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /gdstype |
| concepts[method].geo_series_type:genome-binding-occupancy-profiling-by-high-throughput-sequencing | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /gdstype |
| concepts[modality].local:modality:imaging | enrichment · NCBI GEO | keyword-concept-rules@1.0.0 | title+description (75%) |
| concepts[modality].local:modality:single-cell-rna-seq | enrichment · NCBI GEO | keyword-concept-rules@1.0.0 | title+description (65%) |
| concepts[organism].NCBITaxon:10090 | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /taxon |
| description | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /summary |
| publication_date | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| title | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /title |