Omics · study · 2026
Genome-wide occupancy of CRTC1 in neurons
Listed in NCBI GEO
Description
Background
Gene expression regulated by the transcription factor cAMP-response element binding protein (CREB) is modulated by transcriptional coactivators such as CBP/P300 and CREB-regulated transcription coactivator-1 (CRTC1). However, the genome-wide occupancy profile of CRTC1 in neurons is unknown. Aim: We analyzed the genome-wide occupancy profiles of CRTC1 in primary cortical neurons in basal and stimulated conditions after treatment with forskolin and potassium chloride (FK), which induces CRTC1 dephosphorylation, nuclear translocation and binding to promoter regions of target genes (PMID: 29269871).
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Methods: 40 million reads per sample were quality-filtered and aligned to the reference genome (mm10) before input normalization, peak calling and annotation. Results: We identified strong induction of CRTC1 occupancy after treatment with FK at over 2,500 binding sites comprising proximal promoter regions and distal regions, including well-characterized neuronal activity-regulated enhancers (PMID: 20393465). Conclusions: Neuronal activity induces binding of CRTC1 to promoter and enhancer regions of target genes.
Links
Get the data
- GEO FTP directory ftp.ncbi.nlm.nih.gov/geo/series/GSE131nnn/GSE131395 ↗
download · from NCBI GEO
Where it is published
- GEO accession page ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE131395 ↗
landing page · from NCBI GEO
Documentation and papers
- PRJNA543479 ncbi.nlm.nih.gov/bioproject/PRJNA543479 ↗
project · from NCBI GEO
Topics
- Stated by source
- Genome binding/occupancy profiling by high throughput sequencing · Mus musculus
- From keywords
- Life Sciences
Provenance · 1 source records, 7 field assertions
| Source | Key | Last seen | Raw |
|---|---|---|---|
| NCBI GEO | GSE131395 | 15 d ago | JSON v1 |
| Field | Assertion | Extractor | Evidence |
|---|---|---|---|
| access_level | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[field].local:field:life-sciences | mapping · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[method].geo_series_type:genome-binding-occupancy-profiling-by-high-throughput-sequencing | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /gdstype |
| concepts[organism].NCBITaxon:10090 | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /taxon |
| description | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /summary |
| publication_date | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| title | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /title |