<p>Identification of new glia-specific marker genes.</p>

The blue section represents the plane imaged in the immunostaining shown in (F-F’‘‘). <b>(D)</b> Dot plot showing the newly identified glial markers in the adult dataset per glial cluster. “Non-Glial Cells” represent the combined gene expression of all the remaining cells from the whole adult stage dataset from Özel <i>and colleagues</i>, 2021. <b>(E)</b> Schematic representation of the adult optic lobe, indicating the position of the different glial cell types (in pink) and the localization of the neuropils (dark gray). <b>(F-F’‘‘)</b> Immunostaining of the larval (L3) optic lobe showing the localization of Repo, CG32032 and AnxB9 antibodies. <b>(G-G’‘‘)</b> Immunostaining of the adult optic lobe showing the localization of Repo, CG32032 and AnxB9 antibodies.

CB, Central Brain, fg = fenestrated glia, pg = perineurial glia, spg = subperineurial glia, dsg = distal satellite glia, psg = proximal satellite glia, eg = epithelial glia, mg = marginal glia, xgo = outer chiasm glia, xgi = inner chiasm glia, cxg = cortex glia, mng = medulla neuropil glia, rwg = retina wrapping glia. Scale bar = 20 µm. The code used to generate the graphs can be found in <a href="zenodo.org/records/21890456" target="_blank">zenodo.org/records/21890456</a>.</p>