Table 1_Reverse vaccinology and immunoinformatics-driven design of a multiepitope vaccine candidate against Renibacterium salmoninarum.docx

In this study, a multiepitope vaccine candidate against Renibacterium salmoninarum was designed using reverse vaccinology and immunoinformatics approaches. Conserved antigenic proteins shared among three bacterial strains were identified through a systematic screening pipeline that included virulence prediction, essentiality analysis, antigenicity assessment, and exclusion of host homologs.

Selected B-cell, MHC-I, and MHC-II epitopes were assembled into a chimeric construct and combined with conserved flagellin D0/D1 domains as a TLR5-targeting molecular adjuvant. The resulting construct, designated RS-MEVC, exhibited favorable predicted physicochemical and immunological properties, including high antigenicity, non-allergenicity, structural stability, and favorable stereochemical quality. Three-dimensional modeling revealed a predominantly α-helical structure, and structural validation supported the reliability of the refined model.